Using secretion to solve a solubility problem: High-yield expression in Escherichia coli and purification of the bacterial glycoamidase PNGase F

Protein Expression and Purification

Volumn 24, Issue 1, 2002, Pages 90-98

  Loo, Trevor ^a   Patchett, Mark L ^a   Norris, Gillian E ^a   Lott, J Shaun ^a  

^a MASSEY UNIVERSITY   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); CHRYSEOBACTERIUM MENINGOSEPTICUM; ESCHERICHIA COLI; FLAVOBACTERIUM; FLAVOBACTERIUM MENINGOSEPTICUM; NEGIBACTERIA;

AMIDASE; COMPLEMENTARY DNA; GLYCOPEPTIDASE; HYBRID PROTEIN; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN A;

AMINO ACID SEQUENCE; ARTICLE; ENZYMOLOGY; ESCHERICHIA COLI; FLAVOBACTERIUM; GENE EXPRESSION; GENETICS; GLYCOSYLATION; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SECRETION; SOLUBILITY;

AMIDOHYDROLASES; AMINO ACID SEQUENCE; BACTERIAL OUTER MEMBRANE PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; FLAVOBACTERIUM; GENE EXPRESSION; GLYCOSYLATION; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

EID: 0035996719     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1555     Document Type: Article

References (21)

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2. Requirements of cleavage of high mannose oligosaccharides in glycoproteins by peptide N-glycosidase F
3. Peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase F cannot release glycans with fucose attached α1→3 to the asparagine-linked N-acetylglucosamine residue
4. The three-dimensional structure of PNGase F, a glycosyl-asparaginase from Flavobacterium meningosepticum
5. 4-(N-acetyl-β-D-glucosaminyl)asparagine amidase F at 2.2Å resolution
6. 4-(N-acetyl-β-D-glucosaminyl)asparagine amidase F
7. Purification and crystallization of the endoglycosidase PNGase F, a peptide:N-glycosidase from Flavobacterium meningosepticum
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