Development of a multiplex real-time reverse transcriptase-polymerase chain reaction for equine infectious anemia virus (EIAV)

Journal of Virological Methods

Volumn 105, Issue 1, 2002, Pages 171-179

  Cook, R Frank ^a   Cook, S J ^a   Li, F ^b   Montelaro, R C ^b   Issel, C J ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Dual labelled fluorogenic probes; EIAV; Quantitation of viral load; Real time PCR

Indexed keywords

NUCLEIC ACID; VIRUS RNA; FLUORESCENT DYE;

ANALYTIC METHOD; ARTICLE; CALCULATION; CONTROLLED STUDY; EQUINE INFECTIOUS ANEMIA VIRUS; HORSE; HORSE DISEASE; INTERMETHOD COMPARISON; NONHUMAN; PLASMA; PRIORITY JOURNAL; QUANTITATIVE DIAGNOSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS DIAGNOSIS; ANIMAL; BLOOD; DNA PROBE; EVALUATION; GENE DOSAGE; GENETICS; ISOLATION AND PURIFICATION; PHYSIOLOGY; REPRODUCIBILITY; VIROLOGY; VIRUS LOAD;

ANIMAL; DNA PROBES; EQUINE INFECTIOUS ANEMIA; FLUORESCENT DYES; GENE DOSAGE; HORSES; INFECTIOUS ANEMIA VIRUS, EQUINE; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; VIRAL LOAD; ANIMALS;

EQUIDAE; EQUINE INFECTIOUS ANEMIA VIRUS;

EID: 0035993315     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(02)00101-5     Document Type: Article

References (28)

1. Equine infectious anemia virus: Immunopathogenesis and persistence
2. Equine infectious anemia
3. Development and characterization of an in vivo pathogenic molecular clone of equine infectious anemia virus
4. Differential responses of Equus caballus and Equus asinus to infection with two pathogenic strains of equine infectious anemia virus
5. Longitudinal assessment of feline immunodeficiency virus kinetics in plasma by use of a quantitative competitive reverse transcriptase PCR
6. Zwischenmolekulare energiewanderung und Fluoreszenz
7. Responses in horses infected with equine infectious anemia virus adapted to tissue culture
8. Tissue sites of persistent infection and active replication of equine infectious anemia virus during acute disease and asymptomatic infection in experimentally infected equids
9. DH82 cells: A macrophage cell line for replication and study of equine infectious anemia virus
10. Efficacy of inactivated whole-virus and subunit vaccines in preventing infection and disease caused by equine infectious anemia virus
11. Titration of equine infectious anemia virus. Effect of dosage on incubation time and clinical signs
12. Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: Influence of mismatches on quantification
13. Influence of preassay and sequence variations on viral load determination by a multiplex real-time reverse transcriptase-polymerase chain reaction for feline immunodeficiency virus
14. Viremia and immunological responses in horses infected with equine infectious anemia
15. The S2 gene of equine infectious anemia is a highly conserved determinant of viral replication and virulence properties in experimentally infected ponies
16. Replication in vitro and in vivo of an equine infectious anemia virus mutant deficient in dUTPase activity
17. Production of equine infectious anemia antigen in a persistently infected cell line
18. Immunofluorescent localization of equine infectious anemia virus in tissue
19. Equine retroviruses
20. Virulence and in vitro growth of a cell-adapted strain of equine infectious anemia virus after serial passage in ponies
21. Characterization of infectious molecular clones of equine infectious anemia virus
22. Disease induction by virus derived from molecular clones of equine infectious anemia virus
23. Quantitative competitive polymerase chain reaction for accurate quantitation of HIV DNA and RNA species
24. Viral DNA in horses infected with equine infectious anemia virus
25. In vitro isolation of a neutralization escape mutant of equine infectious anemia virus (EIAV)
26. A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA
27. The immunopathogenesis of equine infectious anemia virus
28. Enhancement of EIAV replication and disease by immunization with a baculovirus-expressed recombinant envelope surface glycoprotein