The amplicon-plus system for high-level expression of transgenes in plants

Nature Biotechnology

Volumn 20, Issue 6, 2002, Pages 622-625

  Mallory, Allison C ^a   Parks, Graham ^a   Endres, Matthew W ^a   Baulcombe, David ^b   Bowman, Lewis H ^a   Pruss, Gail J ^a   Vance, Vicki B ^a  

^a UNIVERSITY OF SOUTH CAROLINA   (United States)

^b SAINSBURY LABORATORY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENES; PLANTS (BOTANY); RNA; TOBACCO; VIRUSES;

AMPLICON-PLUS SYSTEMS;

BIOTECHNOLOGY;

BETA GLUCURONIDASE; VIRUS VECTOR; CYSTEINE PROTEINASE; HC PRO PROTEIN, POTYVIRUS; HC-PRO PROTEIN, POTYVIRUS; VIRUS PROTEIN;

AMPLICON; ARTICLE; BIOTECHNOLOGY; ENZYME ACTIVITY; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSCRIPTION; PRIORITY JOURNAL; RNA REPLICATION; RNA VIRUS; TRANSGENIC PLANT; COMPARATIVE STUDY; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETICS; IMMUNOLOGY; METHODOLOGY; PLANT LEAF; RNA INTERFERENCE; TOBACCO; TRANSGENE;

COMPARATIVE STUDY; CYSTEINE ENDOPEPTIDASES; GENE EXPRESSION; GENE EXPRESSION REGULATION, PLANT; GENETIC ENGINEERING; GLUCURONIDASE; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; RNA INTERFERENCE; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.; TOBACCO; TRANSGENES; VIRAL PROTEINS;

ARABIDOPSIS; ARABIDOPSIS THALIANA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; POTATO VIRUS X; RNA VIRUSES; SOLANUM TUBEROSUM; TOBACCO ETCH VIRUS;

EID: 0035984721     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0602-622     Document Type: Article

References (20)

1. Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA
2. Technical advance: Potato virus X amplicon-mediated silencing of nuclear genes
3. Potato virus X amplicons in Arabidopsis mediate genetic and epigenetic gene silencing
4. Gene silencing as an adaptive defence against viruses
5. RNA silencing in plants-defense and counterdefense
6. A viral suppressor of gene silencing in plants
7. A counterdefensive strategy of plant viruses: Suppression of posttranscriptional gene silencing
8. HC-Pro suppression of transgene silencing eliminates the small RNAs but not transgene methylation or the mobile signal
9. A calmodulin-related protein that suppresses posttranscriptional gene silencing in plants
10. Expression of potyviral polyproteins in transgenic plants reveals three proteolytic activities required for complete processing
11. The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants
12. Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionally
13. A viral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana
14. Plant viral synergism: The potyviral genome encodes a broad-range pathogenicity enhancer that transactivates replication of heterologous viruses
15. Cell-to-cell movement of potexviruses: Evidence for a ribonucleoprotein complex involving the coat protein and first triple gene block protein
16. RNA-based silencing strategies in plants
17. Mutational analysis of the tobacco etch potyviral 35-kDa proteinase: Identification of essential residues and requirements for autoproteolysis
18. Engineering chloroplasts: An alternative site for foreign genes, proteins, reactions and products
19. Milestones in chloroplast genetic engineering: An environmentally friendly era in biotechnology
20. Evidence that the potyvirus P 1 proteinase functions in trans as an accessory factor for genome amplification