Purification of cytochromes P-450scc and P-45017α by steroid-binding affinity column chromatography

Journal of Biochemical and Biophysical Methods

Volumn 49, Issue 1-3, 2001, Pages 417-431

  Kuwada, Masahiro ^a   Sugano, Sachiko ^b   Maki, Jun ^a  

^a KITASATO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b KITASATO UNIVERSITY   (Japan)

Author keywords

DOC: Deoxycorticosterone; EAH Sepharose 4B: 1,6 diaminohexyl Sepharose 4B; Emulgen 913; P 45017 : cytochrome P 45017 ; P 450scc: cytochrome P 450scc; Polyoxyethylenenonylphenylether; SDS: sodium dodecyl sulfate

Indexed keywords

AMIDE; AMINO ACID; CHOLESTEROL; CHOLESTEROL MONOOXYGENASE (SIDE CHAIN CLEAVING); CYTOCHROME P450; CYTOCHROME P450 17ALPHA; CYTOCHROME P450 ISOENZYME; DEOXYCORTICOSTERONE; DODECYL SULFATE SODIUM; ELTANOLONE; LIGAND; SUCCINIC ANHYDRIDE; UNCLASSIFIED DRUG;

ABSORPTION SPECTROPHOTOMETRY; ADRENAL GLAND; AFFINITY CHROMATOGRAPHY; ARTICLE; CATTLE; COLUMN CHROMATOGRAPHY; GEL; HORMONE BINDING; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SWINE; TESTIS;

ADRENAL GLANDS; ANIMALS; BINDING SITES; CATTLE; CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME; CHROMATOGRAPHY, AFFINITY; INDICATORS AND REAGENTS; MALE; STEROID 17-ALPHA-HYDROXYLASE; STEROIDS; SWINE; TESTIS;

EID: 0035975889     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(01)00210-X     Document Type: Article

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