Cocrystal structure of a tRNA Ψ55 pseudouridine synthase: Nucleotide flipping by an RNA-modifying enzyme

Cell

Volumn 107, Issue 7, 2001, Pages 929-939

  Hoang, Charmaine ^a   Ferré D'Amaré, Adrian R ^a  

^a Fred Hutchinson Cancer Research Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID TRANSFER RNA LIGASE; CHAPERONE; NUCLEOTIDE; PROTEIN TRUB; PSEUDOURIDINE; RIBOSOME RNA; RNA; SMALL NUCLEAR RNA; SMALL NUCLEOLAR RIBONUCLEOPROTEIN; TRANSFER RNA; UNCLASSIFIED DRUG; URIDINE;

ARTICLE; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; EUKARYOTE; GENE DISRUPTION; HOMEOBOX; ISOMERISM; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEIN RNA BINDING; PROTEIN TERTIARY STRUCTURE; RNA SEQUENCE; SEQUENCE HOMOLOGY;

ESCHERICHIA COLI; EUKARYOTA; NEGIBACTERIA;

EID: 0035966271     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(01)00618-3     Document Type: Article

References (48)

1. The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of Ψ55 in both mitochondrial and cytoplasmic tRNAs
2. Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the TΨ-loop of yeast tRNAs
3. Enzymatic removal of oxygen for polarography and related methods
4. Crystallography and NMR system: A new software system for macromolecular structure determination
5. Ribbons
6. tRNA recognition by aminoacyl tRNA synthetases
7. Uracil-DNA glycosylase acts by substrate autocatalysis
8. Primary, secondary and tertiary structures of tRNA
9. Preparation of selenomethionyl proteins for phase determination
10. The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I
11. Do proteins predate DNA?
12. Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs
13. Phe
14. Enzymatic formation of modified nucleosides in tRNA: Dependence on tRNA architecture
15. Molecular recognition of tRNA by tRNA pseudouridine 55 synthase
16. The mechanism of pseudouridine synthase I as deduced from its interaction with 5-fluorouracil-tRNA
17. Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes
18. Deletion of the Escherichia coli pseudouridine synthase gene truB blocks formation of pseudouridine 55 in tRNA in vivo, does not affect exponential growth, but confers a strong selective disadvantage in competition with wild-type cells
19. X-linked dyskeratosis congenita is caused by mutations in a highly conserved gene with putative nucleolar functions
20. Protein structure comparison by alignment of distance matrices
21. A conserved aspartate of tRNA pseudouridine synthase is essential for activity and a probable nucleophilic catalyst
22. Improved methods for building protein models in electron density maps and the location of errors in these models
23. 1.9 Å resolution refined structure of TBP recognizing the minor groove of TATAAAAG
24. Small nucleolar RNA-guided post-transcriptional modification of cellular RNAs
25. HhaI methyltransferase flips its target base out of the DNA helix
26. Pseudouridine synthases: Four families of enzymes containing a putative uridine-binding motif also conserved in dUTPases and dCTP deaminases
27. Phe TΨC domain
28. The genomic tag hypothesis: Modern viruses as molecular fossils of ancient strategies for genomic replication
29. A box H/ACA small nucleolar RNA-like domain at the human telomerase 3′ end
30. A telomerase component is defective in the human disease dyskeratosis congenita
31. GRASP-graphical representation and analysis of surface properties
32. RNA processing in microinjected Xenopus oocytes sequential addition of base modifications in a spliced transfer RNA
33. Phe, EF-Tu and a GTP analog
34. Purification, cloning, and properties of the tRNA Ψ55 synthase from Escherichia coli
35. Processing of X-ray diffraction data collected in oscillation mode
36. Base flipping
37. Gln at 2.8 Å resolution: Implications for tRNA discrimination
38. The RNA modification database: 1999 Update
39. Crystal structure of a hairpin ribozyme-inhibitor complex with implications for catalysis
40. Interactions of transfer RNA pseudouridine synthases with RNAs substituted with fluorouracil
41. Database of homology-derived protein structures and structural meaning of sequence alignment
42. The crystal structure of yeast phenylalanine tRNA at 1.93 Å resolution: A classic structure revisited
43. Critical aspartic acid residues in pseudouridine synthases
44. The RNA component of telomerase is mutated in autosomal dominant dyskeratosis congenita
45. Evolutionary appearance of genes encoding proteins associated with box H/ACA snoRNAs: Cbf5p in Euglena gracilis, an early diverging eukaryote, and candidate Gar1p and Nop10p homologs in archaebacteria
46. Crystal structures of restrictocin-inhibitor complexes with implications for RNA recognition and base flipping
47. Modifications of U2 snRNA are required for snRNP assembly and pre-mRNA splicing
48. Point mutations in yeast CBF5 can abolish in vivo pseudouridylation of rRNA