Specific detection of cyclobutane pyrimidine dimers in phytoplankton DNA by a non-radioactive assay based on T4-endonuclease V digestion

Science of the Total Environment

Volumn 277, Issue 1-3, 2001, Pages 149-159

  Fafanel, Maja ^a   Bihari, Nevenka ^a   Krajcar, Valter ^a   Müller, Werner E G ^b   Zahn, Rudolf K ^c   Batel, Renato ^a  

^a RUDER BO KOVI INSTITUTE   (Croatia)

^b JOHANNES GUTENBERG UNIVERSITY   (Germany)

^c Academy of Sciences and Literature   (Germany)

Author keywords

Alkaline filter elution; Crude oil; Cyclobutane pyrimidine dimer (CPD); DNA damage; DNA repair; Isochrysis galbana; Phytoplankton; Sunlight; T4 endonuclease V; UV light

Indexed keywords

ASSAYS; CELL CULTURE; DNA; ELECTROPHORESIS; MARINE BIOLOGY; SUN; ULTRAVIOLET RADIATION;

WATER-SOLUBLE CRUDE OIL FRACTION (WSOF);

ENVIRONMENTAL ENGINEERING;

CYCLOBUTANE; DNA; DYE; ENDONUCLEASE; PETROLEUM; PIGMENT; PYRIMIDINE DIMER; THYROXINE; WATER;

PHYTOPLANKTON;

AGAR GEL ELECTROPHORESIS; ARTICLE; CELL CULTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DAMAGE; DNA DETERMINATION; DNA REPAIR; ENZYME ACTIVITY; HUMAN; HUMAN CELL; MITOSIS INHIBITION; NONHUMAN; PHYTOPLANKTON; PRIORITY JOURNAL; SUN EXPOSURE; ULTRAVIOLET IRRADIATION;

BIOLOGICAL ASSAY; DEOXYRIBONUCLEASE (PYRIMIDINE DIMER); DNA DAMAGE; DNA REPAIR; ENDODEOXYRIBONUCLEASES; PETROLEUM; PHYTOPLANKTON; PYRIMIDINE DIMERS; SUNLIGHT; ULTRAVIOLET RAYS; VIRAL PROTEINS;

ISOCHRYSIS GALBANA;

EID: 0035965029     PISSN: 00489697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0048-9697(00)00870-6     Document Type: Article

References (27)

1. Monitoring ultraviolet-b-induced DNA damage in individual diatom cells by immunofluorescent thymine dimer detection
2. Wavelength-dependant induction of thymine dimers and growth rate reduction in the marine diatom Cyclotella sp. exposed to ultraviolet radiation
3. Nonradiometric detection of DNA crosslinks in mussel hemolymph by alkaline elution
4. Plant response to solar ultraviolet radiation
5. Method for quantitating oil directly in water by fluorescence spectrophotometry
6. Quantitation of radiation-, chemical-, or enzyme-induced single-strand breaks in nonradioactive DNA by alkaline gel electrophoresis: Application to pyrimidine dimers
7. The impact of solar variability on climate
8. Basic radiobiology
9. Ambient solar radiation-induced photodamage in marine bacterioplankton
10. Cell survival characteristics and molecular responses of Antarctic phytoplankton to ultraviolet-B radiation
11. The fluorometric measurement of DNA in animal tissues with special reference to the central nervous system
12. Measurement of strand breaks and cross-links by alkaline elution
13. Benzo(a)pyrene monooxygenase induction in marine fish-molecular response to oil pollution
14. 4-endonuclease V. Perspectives on catalysis
15. Variation in normal and tumour tissue sensitivity of mice to ionising radiation-induced DNA strand breaks in vivo
16. The biology of the (6-4) photoproduct
17. Ultraviolet-b radiation and bacterial metabolism in coastal waters
18. 4-endonuclease V
19. DNA base sequence changes induced by ultraviolet light mutagenesis of a gene on a chromosome in Chinese hamster ovary cells
20. Fluorometric assay using dimeric dyes for double-and single-stranded DNA and RNA with picogram sensitivity
21. Repair systems for nuclear and chloroplast DNA in Chlamydomonas reinhardtii
22. Repair of pyrimidine dimers in ultraviolet-irradiated Chlamydomonas
23. Inhibitory effect of solar radiation on thymidine and leucine incorporation by freshwater and marine bacterioplankton
24. Fluorometric quantitation of single-stranded DNA: A method applicable to the technique of alkaline elution
25. Influence of dispersant on bioavailability of naphthalene from the water-accommodated fraction crude oil to the golden-brown algae Isochrysis galbana
26. DNA alterations by the impact of pollution and problems of risk quantification