A simple method for midkine purification by affinity chromatography with a heavy chain variable domain (VH) fragment of antibody

Journal of Biochemical and Biophysical Methods

Volumn 48, Issue 1, 2001, Pages 77-84

  Dansithong, Warunee ^a   Paul, Sharan ^a   Kojima, Yoshifumi ^a   Kamiya, Kouichirou ^a   Shinozawa, Takao ^a  

^a GUNMA UNIVERSITY   (Japan)

Author keywords

Affinity chromatography; Midkine (MK); Protein purification; Variable heavy chain (VH)

Indexed keywords

BUFFER; DNA FRAGMENT; MIDKINE; MONOCLONAL ANTIBODY; SEPHAROSE; SODIUM CHLORIDE;

AFFINITY CHROMATOGRAPHY; ANTIBODY PRODUCTION; ARTICLE; BINDING AFFINITY; CELL LYSATE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HYBRIDOMA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; CARRIER PROTEINS; CHROMATOGRAPHY, AFFINITY; CYTOKINES; ESCHERICHIA COLI; GENETIC VECTORS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 0035962078     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(00)00145-7     Document Type: Article

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