Design and construction of a Haemophilus influenzae conjugal expression system

Gene

Volumn 281, Issue 1-2, 2001, Pages 95-102

  Daines, Dayle A ^a   Smith, Arnold L ^a  

^a University of Missouri   (United States)

Author keywords

Conjugation; Genetic system; Promoter; Vector

Indexed keywords

ANTIBIOTIC AGENT;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL INFECTION; CELL LINE; CHROMOSOME; CONJUGATION; CONTROLLED STUDY; ESCHERICHIA COLI; EXPERIMENTAL MODEL; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; GENE TRANSFER; GENETIC CODE; HAEMOPHILUS INFLUENZAE; HOST; IN VITRO STUDY; IN VIVO STUDY; MARKER GENE; METHODOLOGY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; TISSUE CULTURE;

BLOTTING, WESTERN; CHLORAMPHENICOL O-ACETYLTRANSFERASE; CONJUGATION, GENETIC; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; HAEMOPHILUS INFLUENZAE; HISTIDINE; LAC OPERON; OPERON; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; TRYPTOPHAN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HAEMOPHILUS; HAEMOPHILUS INFLUENZAE; NEGIBACTERIA; VECTORS;

EID: 0035960918     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(01)00788-0     Document Type: Article

References (26)

1. Transfer of Haemophilus influenzae chromosomal genes by cell-to-cell contact
2. Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas
3. Effects of genes exerting growth inhibition and plasmid stability on plasmid maintenance
4. Spacing of the -10 and -35 regions in the tac promoter: Effect on its in vivo activity
5. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
6. Vectors with restriction site banks V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites
7. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd
8. Molecular nature of two nonconjugative plasmids carrying drug resistance genes
9. Transformation of Mycobacterium aurum and Mycobacterium smegmatis with the broad-host-range gram-negative cosmid vector pJRD215
10. Construction and characterization of a recA mutant of Thiobacillus ferrooxidans by marker exchange mutagenesis
11. Cloning of the rec-2 locus of Haemophilus influenzae
12. Cloning, sequencing, and expression of the gene encoding the high-molecular-weight cytochrome c from Desulfovibrio vulgaris Hildenborough
13. Small broad-host-range gentamicin resistance gene cassettes for site-specific insertion and deletion mutagenesis
14. Intracellular multiplication and human macrophage killing by Legionella pneumophila are inhibited by conjugal components of the IncQ plasmid RSF1010
15. A complex of recombination and repair genes in Haemophilus influenzae
16. A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in Gram-negative bacteria
17. Plasmid transfer in Haemophilus influenzae
18. Improved production of poly(4-hydroxybutyrate) by Comamonas acidovorans and its freeze-fracture morphology
19. Conjugative transfer in the dissemination of beta-lactam and aminoglycoside resistance
20. Isolation and characterization of mutants of Haemophilus influenzae deficient in an adenosine 5′-triphosphate-dependent deoxyribonuclease activity
21. Transformation of Actinomyces spp. by a gram-negative broad-host-range plasmid
22. Production of eicosapentenoic acid by a recombinant marine cyanobacterium, Synechococcus sp.