Specific DNA binding and transactivation potential of recombinant, purified Stat5

Molecular and Cellular Endocrinology

Volumn 183, Issue 1-2, 2001, Pages 101-112

  Beisenherz Huss, Christian ^a   Mundt, Maren ^b   Herrala, Annakaisa ^c   Vihko, Pirkko ^c   Schubert, Alexis ^b   Groner, Bernd ^b  

^a UNIVERSITY OF FREIBURG   (Germany)

^b INSTITUTE FOR TUMOR BIOLOGY AND EXPERIMENTAL THERAPY   (Germany)

^c UNIVERSITY OF OULU   (Finland)

Author keywords

Phosphatase treatment; Phosphorylation; Recombinant Stat5; Transactivation

Indexed keywords

PHOSPHATASE; PHOSPHOTRANSFERASE; RECOMBINANT PROTEIN; STAT5 PROTEIN; STAT5B PROTEIN; TYROSINE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA BINDING; IMMUNOBLOTTING; INSECT; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; TRANSACTIVATION;

ANIMALS; CHROMATOGRAPHY; COS CELLS; DNA; DNA-BINDING PROTEINS; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICROINJECTIONS; MILK PROTEINS; PHOSPHORYLATION; PROTEIN-TYROSINE-PHOSPHATASE; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECEPTORS, PLATELET-DERIVED GROWTH FACTOR; RECOMBINANT PROTEINS; STAT5 TRANSCRIPTION FACTOR; TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS; TYROSINE;

EID: 0035950439     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0303-7207(01)00588-3     Document Type: Article

References (36)

1. Interleukin-3 signals through multiple isoforms of Stat5
2. Interleukin-2 activation of STAT5 requires the convergent action of tyrosine kinases and a serine/threonine kinase pathway distinct from the Raf1/ERK2 MAP kinase pathway
3. Stat5a serine phosphorylation. Serine 779 is constitutively phosphorylated in the mammary gland, and serine 725 phosphorylation influences prolactin-stimulated in vitro DNA binding activity
4. Insulin stimulates the serine phosphorylation of the signal transducer and activator of transcription (STAT3) isoform
5. STAT3 serine phosphorylation by ERK-dependent and -independent pathways negatively modulates its tyrosine phosphorylation
6. Transcriptional regulation of the junB promoter: analysis of STAT-mediated signal transduction
7. Serine phosphorylation of STATs
8. Identification of JAK protein tyrosine kinases as signaling molecules for prolactin. Functional analysis of prolactin receptor and prolactin-erythropoietin receptor chimera expressed in lymphoid cells
9. Activation of Jak2 catalytic activity requires phosphorylation of Y1007 in the kinase activation loop
10. Steel factor induces serine phosphorylation of Stat3 in human growth factor-dependent myeloid cell lines
11. Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription
12. Ha-ras and v-raf oncogenes, but not int-2 and c-myc, interfere with the lactogenic hormone dependent activation of the mammary gland specific transcription factor
13. Two discrete regions of interleukin-2 (IL-2) receptor beta independently mediate IL-2 activation of a PD98059/rapamycin/wortmannin-insensitive Stat5a/b serine kinase
14. Stat1 combines signals derived from IFN-gamma and LPS receptors during macrophage activation
15. Elf-1 and Stat5 bind to a critical element in a new enhancer of the human interleukin-2 receptor alpha gene
16. Stat5a is mandatory for adult mammary gland development and lactogenesis
17. CIS, a cytokine inducible SH2 protein, is a target of the JAK-STAT5 pathway and modulates STAT5 activation
18. Thrombopoietin-induced differentiation of a human megakaryoblastic leukemia cell line, CMK, involves transcriptional activation of p21(WAF1/Cip1) by STAT5
19. Deletion of the carboxyl-terminal transactivation domain of MGF-Stat5 results in sustained DNA binding and a dominant negative phenotype
20. Interleukin-3, granulocyte-macrophage colony-stimulating factor, and interleukin-5 transduce signals through two forms of STAT5
21. Protein interactions at the carboxyl terminus of p53 result in the induction of its in vitro transactivation potential
22. Beta-casein gene promoter activity is regulated by the hormone-mediated relief of transcriptional repression and a mammary-gland-specific nuclear factor
23. STAT-like DNA-binding activity in Spodoptera frugiperda cells
24. Cytokine signaling through non-receptor protein tyrosine kinases
25. Stat5a and Stat5b proteins have essential and non-essential, or redundant, roles in cytokine responses
26. A sequence of the CIS gene promoter interacts preferentially with two associated STAT5A dimers: a distinct biochemical difference between STAT5A and STAT5B
27. Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response
28. Mammary gland-specific nuclear factor is present in lactating rodent and bovine mammary tissue and composed of a single polypeptide of 89 kDa
29. Lactogenic hormone activation of Stat5 and transcription of the beta-casein gene in mammary epithelial cells is independent of p42 ERK2 mitogen-activated protein kinase activity
30. Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation
31. Specificity of transcription enhancement via the STAT responsive element in the serine protease inhibitor 2.1 promoter
32. Differential control of the phosphorylation state of proline-juxtaposed serine residues Ser725 of Stat5a and Ser730 of Stat5b in prolactin-sensitive cells
33. Serine phosphorylation and maximal activation of STAT3 during CNTF signaling is mediated by the rapamycin target mTOR
34. Mouse oncostatin M: an immediate early gene induced by multiple cytokines through the JAK-STAT5 pathway
35. Requirement of serine phosphorylation for formation of STAT-promoter complexes
36. Stat1 serine phosphorylation occurs independently of tyrosine phosphorylation and requires an activated Jak2 kinase