The Mechanism of Substrate Recognition of Pyroglutamyl-peptidase I from Bacillus amyloliquefaciens as Determined by X-ray Crystallography and Site-directed Mutagenesis

Journal of Biological Chemistry

Volumn 276, Issue 21, 2001, Pages 18557-18562

  Ito, Kiyoshi ^a   Inoue, Takahiko ^a   Takahashi, Tomoyuki ^a   Huang, Hua Shan ^a   Esumi, Tomoyuki ^a   Hatakeyama, Susumi ^a   Tanaka, Nobutada ^b   Nakamura, Kazuo T ^b   Yoshimoto, Tadashi ^a  

^a NAGASAKI UNIVERSITY   (Japan)

^b SHOWA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BACTERIA; CATALYST ACTIVITY; CRYSTAL STRUCTURE; ENZYME KINETICS; HYDROGEN BONDS; HYDROPHOBICITY; MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

MUTATIONS;

BIOCHEMISTRY;

2 PYRROLIDONE DERIVATIVE; 5 OXOPROLYL PEPTIDASE; AMINOPEPTIDASE; ENZYME INHIBITOR; ISOLEUCINE; PHENYLALANINE; THREONINE; VALINE;

AMINO ACID COMPOSITION; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS AMYLOLIQUEFACIENS; CRYSTAL STRUCTURE; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; GENE INSERTION; GENE MUTATION; HYDROGEN BOND; HYDROPHOBICITY; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; MUTANT; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; WILD TYPE; X RAY CRYSTALLOGRAPHY; AMINO ACID SEQUENCE; BACILLUS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN FOLDING; SEQUENCE ALIGNMENT; STRUCTURE ACTIVITY RELATION;

BACILLUS AMYLOLIQUEFACIENS; INSERTION SEQUENCES; POSIBACTERIA;

AMINO ACID SEQUENCE; BACILLUS; CRYSTALLOGRAPHY, X-RAY; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN FOLDING; PYROGLUTAMYL-PEPTIDASE I; SEQUENCE ALIGNMENT; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 0035947667     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M011724200     Document Type: Article

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