Duplication and extension of the Thorneley and Lowe kinetic model for Klebsiella pneumoniae nitrogenase catalysis using a MATHEMATICA software platform

Biophysical Chemistry

Volumn 91, Issue 3, 2001, Pages 281-304

  Wilson, P E ^a   Nyborg, A C ^a   Watt, G D ^a  

^a Brigham Young University   (United States)

Author keywords

Catalytic efficiency; Computer simulation; Nitrogenase kinetics; Product distribution

Indexed keywords

NITROGENASE;

ARTICLE; CATALYSIS; COMPUTER PROGRAM; ELECTRON TRANSPORT; ENZYME KINETICS; HYDROLYSIS; KLEBSIELLA PNEUMONIAE; NITROGEN FIXATION; NONHUMAN; OXIDATION REDUCTION STATE; PRIORITY JOURNAL;

ADENOSINE TRIPHOSPHATE; CATALYSIS; IRON; KINETICS; KLEBSIELLA PNEUMONIAE; MODELS, CHEMICAL; MOLYBDENUM; NITROGENASE; PHOSPHATES; SOFTWARE;

KLEBSIELLA PNEUMONIAE;

EID: 0035942982     PISSN: 03014622     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0301-4622(01)00182-X     Document Type: Article

References (38)

1. The nitrogen-fixing complex of bacteria
2. Metalloclusters of the nitrogenases
3. Nitrogenase and biological nitrogen fixation
4. Mechanism of molybdenum nitrogenase
5. Kinetics and mechanism of the nitrogenase enzyme system
6. Substrate reactions of nitrogenase
7. Redox reactions of and nucleotide binding to the iron protein of Azotobacter vinelandii
8. Kinetic studies of nitrogenase from soybean root nodule bacteroids
9. Nitrogenase and nitrogenase reductase associate and dissociate with each catalytic cycle
10. Components and pathways in biological nitrogen fixation
11. Mechanistic interpretation of the dilution effect for Azotobacter vinelandii and Clostridium pasteurianum nitrogenase catalysis
12. Nitrogenase of Klebsiella pneumoniae: kinetics of the dissociation of oxidized iron protein from molybdenum iron protein. Identification of the rate-limiting step for substrate reduction
13. 2 evolution rate on component-protein concentration and ratio and sodium dithionite concentration
14. 3 formation
15. 2 evolution
16. 2 formation
17. Klebsiella pneumoniae nitrogenase mechanism of acetylene reduction and its inhibition by carbon monoxide
18. Klebsiella pneumoniae nitrogenase pre-steady-state absorbance changes show that redox changes occur in the MoFe protein that depend on substrate and component protein ratio: a role for P-centres in reducing dinitrogen?
19. ATP hydrolysis and energy transduction by nitrogenase
20. Nitrogenase of Klebsiella pneumoniae. A stopped-flow study of magnesium-adenosine triphosphate-induced electron transfer between the component proteins
21. Salt inhibition of nitrogenase catalysis and salt effects on the separate protein components
22. Effect of salts on Azotobacter vinelandii nitrogenase activities: inhibition of iron chelation and substrate reduction
23. 4 cluster in the iron protein component of Azotobacter vinelandii nitrogenase
24. Enhanced efficiency of ATP hydrolysis during nitrogenase catalysis utilizing reductants that form the all-ferrous redox state of the Fe protein
25. Reactions of Azotobacter vinelandii nitrogenase using Ti(III) as reductant
26. A method for preparing analytically pure sodium dithionite. Dithionite quality and observed nitrogenase-specific activities
27. Steady-state kinetic studies of dithionite utilization, component protein interaction, and the formation of an oxidized iron protein intermediate during Azotobacter vinelandii nitrogenase catalysis
28. Nitrogenase of Azotobacter chroococcum: kinetics of the reduction of oxidized iron protein by sodium dithionite
29. Evidence for electron transfer from the nitrogenase iron protein to the molybdenum-iron protein without MgATP hydrolysis: characterization of a tight protein-protein complex
30. Nitrogenase of Klebsiella pneumoniae: reductant-independent ATP hydrolysis and the effects of pH on the efficiency of coupling of ATP hydrolysis to substrate reduction
31. Reductant-independent ATP hydrolysis catalyzed by homologous nitrogenase proteins from Azotobacter vinelandii and heterologous crosses with Clostridium pasteurianum
32. Klebsiella pneumoniae nitrogenase: the pre-steady-state kinetics of MoFe-protein reduction and hydrogen evolution under conditions of limiting electron flux show that the rates of association with the Fe-protein and electron transfer are independent of the oxidation level of the MoFe-protein
33. Hydrogen burst associated with nitrogenase-catalyzed reactions
34. Nitrogenase reactivity effects of component ratio on electron flow and distribution during nitrogen fixation
35. Stoichiometry, ATP/2e values, and energy requirements for reactions catalyzed by nitrogenase from Azotobacter vinelandii
36. Nitrogenase of Klebsiella pneumoniae: reversibility of the reductant-independent magnesium ATP-cleavage reaction is shown by magnesium ADP-catalyzed phosphate-water oxygen exchange
37. Nitrogenase
38. Nitrogenase electron transfer and allocation and the role of ATP