Correlation between steady-state ATP hydrolysis and Vanadate-induced ADP trapping in human P-glycoprotein: Evidence for ADP release as the rate-limiting step in the catalytic cycle and its modulation by substrates

Journal of Biological Chemistry

Volumn 276, Issue 12, 2001, Pages 8657-8664

  Kerr, Kathleen M ^a   Sauna, Zuben E ^c   Ambudkar, Suresh V ^b  

^a US Patent and Trademark Office   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c NONE

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINETRIPHOSPHATE; BIOASSAY; CATALYST ACTIVITY; CELLS; HYDROLYSIS; PROTEINS;

MULTIDRUG RESISTANCE;

BIOCHEMISTRY;

8 AZIDOADENOSINE DIPHOSPHATE; 8 AZIDOADENOSINE TRIPHOSPHATE; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE DERIVATIVE; CYCLOSPORIN A; CYTOTOXIC AGENT; GLYCOPROTEIN P; MEMBRANE PROTEIN; NUCLEOTIDE; PHOSPHORUS 32; PROTEIN INHIBITOR; RAPAMYCIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VALSPODAR; VANADIC ACID; VERAPAMIL; 8-AZIDOADENOSINE DIPHOSPHATE; AZIDE; DRUG DERIVATIVE;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CATALYSIS; CHEMICAL REACTION; CONTROLLED STUDY; DISSOCIATION; ENZYME ACTIVITY; ENZYME REGULATION; HUMAN; HYDROLYSIS; KINETICS; MOLECULAR PROBE; MULTIDRUG RESISTANCE; NONHUMAN; PHASE TRANSITION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN FUNCTION; PROTEIN METABOLISM; PROTEIN PURIFICATION; REGULATORY MECHANISM; STEADY STATE; METABOLISM;

ANIMALIA;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; AZIDES; CATALYSIS; HUMANS; HYDROLYSIS; KINETICS; P-GLYCOPROTEIN; VANADATES;

EID: 0035937707     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M010044200     Document Type: Article

References (56)

1. Ambudkar, S. V., Dey, S., Hrycyna, C. A., Ramachandra, M., Pastan, I., and Gottesman, M. M. (1999) Annu. Rev. Pharmacol. Toxicol. 39, 361-398
2. Gottesman, M. M., Hrycyna, C. A., Schoenlein, P. V., Germann, U. A., and Pastan, I. (1995) Annu. Rev. Genet. 29, 607-649
3. Gottesman, M. M., and Pastan, I. (1993) Annu. Rev. Biochem. 62, 385-427
4. Croop, J. M. (1998) Methods Enzymol. 292, 101-116
5. Gottesman, M. M., Pastan, I., and Ambudkar, S. V. (1996) Curr. Opin. Genet. Dev. 6, 610-617
6. Ramachandra, M., Ambudkar, S. V., Gottesman, M. M., Pastan, I., and Hrycyna, C. A. (1996) Mol. Biol. Cell 7, 1485-1498
7. Sarkadi, B., Price, E. M., Boucher, R. C., Germann, U. A., and Scarborough, G. A. (1992) J. Biol. Chem. 267, 4854-4858
8. Ambudkar, S. V., Lelong, I. H., Zhang, J., Cardarelli, C. O., Gottesman, M. M., and Pastan, I. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 8472-8476
9. Rao, U. S., and Scarborough, G. A. (1994) Mol. Pharmacol. 45, 773-776
10. Ambudkar, S. V. (1998) Methods Enzymol. 292, 504-514
11. Ramachandra, M., Ambudkar, S. V., Chen, D., Hrycyna, C. A., Dey, S., Gottesman, M. M., and Pastan, I. (1998) Biochemistry 37, 5010-5019
12. Senior, A. E., al-Shawi, M. K., and Urbatsch, I. L. (1998) Methods Enzymol. 292, 514-523
13. Urbatsch, I. L., and Senior, A. E. (1995) Arch. Biochem. Biophys. 316, 135-140
14. Doige, C. A., Yu, X., and Sharom, F. J. (1993) Biochim. Biophys. Acta 1146, 65-72
15. Sharom, F. J. (1997) Biochem. Soc. Trans. 25, 1088-1096
16. Romsicki, Y., and Sharom, F. J. (1999) Biochemistry 38, 6887-6896
17. Liu, R., and Sharom, F. J. (1996) Biochemistry 35, 11865-11873
18. Urbatsch, I. L., Sankaran, B., Weber, J., and Senior, A. E. (1995) J. Biol. Chem. 270, 19383-19390
19. Urbatsch, I. L., Sankaran, B., Bhagat, S., and Senior, A. E. (1995) J. Biol. Chem. 270, 26956-26961
20. Urbatsch, I. L., Beaudet, L., Carrier, I., and Gros, P. (1998) Biochemistry 37, 4592-4602
21. Hrycyna, C. A., Ramachandra, M., Ambudkar, S. V., Ko, Y. H., Pedersen, P. L., Pastan, I., and Gottesman, M. M. (1998) J. Biol. Chem. 273, 16631-16634
22. Senior, A. E., al-Shawi, M. K., and Urbatsch, I. L. (1995) FEBS Lett. 377, 285-289
23. Germann, U. A. (1998) Methods Enzymol. 292, 427-441
24. Schaffner, W., and Weissmann, C. (1973) Anal. Biochem. 56, 502-514
25. Sauna, Z. E., and Ambudkar, S. V. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 2515-2520
26. Ambudkar, S. V., Lelong, I. H., Zhang, J., and Cardarelli, C. (1998) Methods Enzymol. 292, 492-504
27. Germann, U. A., Chambers, T. C., Ambudkar, S. V., Licht, T., Cardarelli, C. O., Pastan, I., and Gottesman, M. M. (1996) J. Biol. Chem. 271, 1708-1716
28. Urbatsch, I. L., al-Shawi, M. K., and Senior, A. E. (1994) Biochemistry 33, 7069-7076
29. Dey, S., Ramachandra, M., Pastan, I., Gottesman, M. M., and Ambudkar, S. V. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 10594-10599
30. Litman, T., Nielsen, D., Skovsgaard, T., Zeuthen, T., and Stein, W. D. (1997) Biochim. Biophys. Acta 1361, 147-158
31. Rebbeor, J. F., and Senior, A. E. (1998) Biochim. Biophys. Acta 1369, 85-93
32. Garrigos, M., Mir, L. M., and Orlowski, S. (1997) Eur. J. Biochem. 244, 664-673
33. Litman, T., Zeuthen, T., Skovsgaard, T., and Stein, W. D. (1997) Biochim. Biophys. Acta 1361, 169-176
34. Litman, T., Zeuthen, T., Skovsgaard, T., and Stein, W. D. (1997) Biochim. Biophys. Acta 1361, 159-168
35. Lee, C. G., Gottesman, M. M., Cardarelli, C. O., Ramachandra, M., Jeang, K. T., Ambudkar, S. V., Pastan, I., and Dey, S. (1998) Biochemistry 37, 3594-3601
36. Rao, U. S. (1995) J. Biol. Chem. 270, 6686-6690
37. Loo, T. W., and Clarke, D. M. (1995) J. Biol. Chem. 270, 21449-21452
38. al-Shawi, M. K., and Senior, A. E. (1993) J. Biol. Chem. 268, 4197-4206
39. al-Shawi, M. K., Urbatsch, I. L., and Senior, A. E. (1994) J. Biol. Chem. 269, 8986-8992
40. Dey, S., Hafkemeyer, P., Pastan, I., and Gottesman, M. M. (1999) Biochemistry 38, 6630-6639
41. Demeule, M., Laplante, A., Murphy, G. F., Wenger, R. M., and Beliveau, R. (1998) Biochemistry 37, 18110-18118
42. Sharom, F. J., Yu, X., Chu, J. W., and Doige, C. A. (1995) Biochem. J. 308, 381-390
43. Shepard, R. L., Winter, M. A., Hsaio, S. C., Pearce, H. L., Beck, W. T., and Dantzig, A. H. (1998) Biochem. Pharmacol. 56, 719-727
44. Szabo, K., Welker, E., Bakos, Muller, M., Roninson, I., Varadi, A., and Sarkadi, B. (1998) J. Biol. Chem. 273, 10132-10138
45. Senior, A. E. (1998) Acta Physiol. Scand. Suppl. 643, 213-218
46. Senior, A. E., and Gadsby, D. C. (1997) Semin. Cancer. Biol. 8, 143-150
47. Saeki, T., Ueda, K., Tanigawara, Y., Hori, R., and Komano, T. (1993) J. Biol. Chem. 268, 6077-6080
48. Schramm, U., Fricker, G., Wenger, R., and Miller, D. S. (1995) Am. J. Physiol. 268, F46-F52
49. Schinkel, A. H., Wagenaar, E., van Deemter, L., Mol, C. A., and Borst, P. (1995) J. Clin. Invest. 96, 1698-1705
50. Didier, A., Wenger, J., and Loor, F. (1995) Anticancer Drugs 6, 669-680
51. Mechetner, E. B., Schott, B., Morse, B. S., Stein, W. D., Druley, T., Davis, K. A., Tsuruo, T., and Roninson, I. B. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 12908-12913
52. Pascaud, C., Garrigos, M., and Orlowski, S. (1998) Biochem. J. 333, 351-358
53. Loo, T. W., and Clarke, D. M. (1997) J. Biol. Chem. 272, 31945-31948
54. Nagata, K., Nishitani, M., Matsuo, M., Kioka, N., Amachi, T., and Ueda, K. (2000) J. Biol. Chem. 275, 17626-17630
55. Sun, H., Molday, R. S., and Nathans, J. (1999) J. Biol. Chem. 274, 8269-8281
56. Sauna, Z. E., and Ambudkar, S. V. (2001) J. Biol. Chem., in press