Purification and characterization of two serine protease inhibitors from the hemolymph of Mythimna unipuncta

Insect Biochemistry and Molecular Biology

Volumn 31, Issue 8, 2001, Pages 761-769

  Cherqui, Anas ^a   Cruz, Norberto ^a   Simões, Nelson ^a  

^a UNIVERSITY OF THE AZORES   (Portugal)

Author keywords

Inhibitor; Insect; Mythimna unipuncta; N terminal sequence; Phenoloxidase; Purification; Serine protease; Serpin

Indexed keywords

CHYMOTRYPSIN; CHYMOTRYPSIN INHIBITOR; INSECT PROTEIN; SERINE PROTEINASE INHIBITOR; TRYPSIN INHIBITOR;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL; ARTICLE; CHEMICAL ANALYSIS; HEMOLYMPH; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; LEPIDOPTERA; MOLECULAR GENETICS; MOLECULAR WEIGHT; MOTH; NONHUMAN; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; TWO DIMENSIONAL ELECTROPHORESIS;

AMINO ACID SEQUENCE; ANIMALS; CHYMOTRYPSIN; HEMOLYMPH; INSECT PROTEINS; MOLECULAR SEQUENCE DATA; MOTHS; SERINE PROTEINASE INHIBITORS; TRYPSIN INHIBITORS;

INSECTA; MYTHIMNA; MYTHIMNA UNIPUNCTA;

EID: 0035933366     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(00)00172-7     Document Type: Article

References (38)

1. Activation of pre-phenoloxidase III. Release of a peptide from pre-phenoloxidase by the activating system
2. Biochemistry of the phenoloxidase system in insects: With special reference to its activation
3. An introduction to the proteinase
4. Insect immunity: Two proteinase inhibitors from hemolymph of Locusta migratoria
5. Secreted proteases from Photorhabdus luminescens: Separation the extracellular proteases from the insecticidal Tc toxin complexes
6. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding
7. Purification of proteinase inhibitor which controls prophenoloxidase activation in hemolymph of Locusta migratoria (Insecta)
8. Purification of the prophenoloxidase from haemolymph of Locusta migratoria
9. Sodium dodecyl sulfate-stable complexes between serpins and active or inactive proteinases contain the region COOH-terminal to the reactive site loop
10. Automated equipment for sequence determinations
11. Properties of protease inhibitors from the haemolymph of silkworms, Bombyx mori, Antheraea pernyi and Philosamia cynthiaricini
12. Purification and characterization of a fungal protease specific protein inhibitor (FPI-F) in the silkworm haemolymph
13. Substrate gel electrophoresis for composition and molecular weight of proteinases or proteinaceous proteinase inhibitors
14. Purification and characterization of a group of five novel peptide serine protease inhibitors from ovaries of the desert locust, Schistocerca gregaria
15. Characterization and functional analysis of 12 naturally occurring reactive site variants of serpin- from Manduca sexta
16. Organization of serpin gene-1 from Manduca sexta
17. Purification and partial characterization of a protease inhibitor from Drosophila melanogaster
18. Isolation and characterization of four serine protease inhibitors (serpins) from hemolymph of Manduca sexta
19. Primary structure of a member of the serpin superfamily of proteinase inhibitors from an insect, Manduca sexta
20. The characterization of enzyme inhibition
21. Cleavage of structural protein during assembly of the head of bacteriophage T4
22. In vitro activation of pro-phenoloxidase by two kinds of pro-phenoloxidase activating factors isolated from hemolymph of coleopteran, Holotrichia diomphalia larvae
23. Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity
24. High resolution two-dimensional electrophoresis of proteins
25. Host age of Pseudalaetia unipuncta (Lepidoptera: Noctuidae) and parasitic capacity of Glyptanteles militaris (Hymenoptera: Braconidae)
26. Analysis of proteins from different phase variants of the entomopathogenic bacteria Photorhabdus luminescens by two dimensional zymography
27. Purification and characterization of two trypsin inhibitors from the hemolymph of Manduca sexta larvae
28. Amino-acid sequences of two basic α-chymotrypsin inhibitors from silkworm larval hemolymph
29. Isolation of two novel proteinase inhibitors from the hemolymph of silkworm larva, Bombyx mori. Comparison with human serum proteinase inhibitors
30. Prophenoloxidase-activating enzyme of the silkworm Bombyx mori. Purification characterization and cDNA cloning
31. Characterization of an extracellular protease from the insect pathogen Xenorhabdus luminescens
32. Purification of α-chymotrypsin inhibitors from the larval hemolymph of the silkworm Bombyx mori
33. The properties and purification of a Blaberus craniifer plasma protein which enhances the activation of the haemocyte prophenoloxidase by a β-1,3 glucan
34. Endogenous protease inhibitors prevent undesired activation of prophenolase in insect hemolymph
35. Amino acid of silkworm (Bombyx mori) hemolymph antitrypsin deduced from its cDNA nucleotide sequence: Confirmation of its homology with serpins
36. Characterization of natural inhibitors of trypsin and chymotrypsin by electrophoresis in acrylamide-agarose gels
37. Cloning of two cDNAs encoding three small serine protease inhibiting peptides from the desert locust Schistocerca gregaria and analysis of tissue-dependent and stage-dependent expression
38. Microbial activation of the two serine enzymes and prophenoloxidase in the plasma fraction of hemolymph of the silkworm Bombyx mori