The Hemophilus influenzae Hap Autotransporter Is a Chymotrypsin Clan Serine Protease and Undergoes Autoproteolysis via an Intermolecular Mechanism

Journal of Biological Chemistry

Volumn 276, Issue 42, 2001, Pages 39492-39500

  Fink, Doran L ^a   Cope, Leslie D ^b,c   Hansen, Eric J ^b   St Geme III, Joseph W ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^c MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BACTERIA; BIOLOGICAL MEMBRANES; CATALYSIS; MUTAGENESIS; PROTEINS;

INTERMOLECULAR MECHANISMS; MICROCOLONY FORMATION;

BIOCHEMISTRY;

ADHESIN; AMINO ACID; ASPARTIC ACID; BACTERIAL PROTEIN; CARRIER PROTEIN; CHYMOTRYPSIN; HISTAMINE; PROTEIN HAP; SERINE PROTEINASE; UNCLASSIFIED DRUG; HAP PROTEIN, HEMOPHILUS INFLUENZAE; HISTIDINE; OUTER MEMBRANE PROTEIN; SERINE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL COLONIZATION; BACTERIUM ADHERENCE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; HAEMOPHILUS INFLUENZAE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN SECRETION; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; BINDING SITE; CATALYSIS; CHEMISTRY; ENZYME ACTIVE SITE; GENETIC PROCEDURES; KINETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; PLASMID; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SEQUENCE HOMOLOGY; TIME; TRANSPORT AT THE CELLULAR LEVEL;

BACTERIA (MICROORGANISMS); HAEMOPHILUS; HAEMOPHILUS INFLUENZAE;

AMINO ACID SEQUENCE; ASPARTIC ACID; BACTERIAL OUTER MEMBRANE PROTEINS; BINDING SITES; BIOLOGICAL TRANSPORT; CATALYSIS; CATALYTIC DOMAIN; CHYMOTRYPSIN; CONSERVED SEQUENCE; GENETIC TECHNIQUES; HAEMOPHILUS INFLUENZAE; HISTIDINE; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLASMIDS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; SERINE ENDOPEPTIDASES; TIME FACTORS;

EID: 0035914443     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M106913200     Document Type: Article

References (42)

1. Henderson, I. R., Cappello, R., and Nataro, J. P. (2000) Trends Microbiol. 8, 529-532
2. Henderson, I. R., and Nataro, J. P. (2001) Infect. Immun. 69, 1231-1243
3. Turk, D. C. (1984) J. Med. Microbiol. 18, 1-16
4. St. Geme, J. W., III, de la Morena, M. L., and Falkow, S. (1994) Mol. Microbiol. 14, 217-233
5. Hendrixson, D. R., and St Geme, J. W., III (1998) Mol. Cell 2, 841-850
6. Hendrixson, D., de la Morena, M. L., Stathopoulos, C., and St. Geme, J. W., III (1997) Mol. Microbiol. 26, 505-518
7. Brunder, W., Schmidt, H., and Karch, H. (1997) Mol. Microbiol. 24, 767-778
8. Eslava, C., Navarro-Garcia, F., Czeczulin, J. R., Henderson, I. R., Cravioto, A., and Nataro, J. P. (1998) Infect. Immun. 66, 3155-3163
9. Henderson, I. R., Czeczulin, J., Eslava, C., Noriega, F., and Nataro, J. P. (1999) Infect. Immun. 67, 5587-5596
10. Stathopoulos, C., Provence, D. L., and Curtiss, R., III (1999) Infect. Immun. 67, 772-781
11. Benjelloun-Touimi, Z., Sansonetti, P. J., and Parsot, C. (1995) Mol. Microbiol. 17, 123-135
12. Stein, M., Kenny, B., Stein, M. A., and Finlay, B. B. (1996) J. Bacteriol. 178, 6546-6554
13. Al-Hasani, K., Henderson, I. R., Sakellaris, H., Rajakumar, K., Grant, T., Nataro, J. P., Robins-Browne, R., and Adler, B. (2000) Infect. Immun. 68, 2457-2463
14. Kilian, M., Mestecky, J., Kulhavy, R., Tomana, M., and Butler, W. T. (1980) J. Immunol. 124, 2596-2600
15. Lin, L., Ayala, P., Larson, J., Mulks, M., Fukuda, M., Carlsson, S. R., Enns, C., and So, M. (1997) Mol. Microbiol. 24, 1083-1094
16. Pohlner, J., Langenberg, U., Wolk, U., Beck, S. C., and Meyer, T. F. (1995) Mol. Microbiol. 17, 1073-1083
17. Setlow, J. K., Brown, D. C., Boling, M. E., Mattingly, A., and Gordon, M. P. (1968) J. Bacteriol. 95, 546-558
18. Tomb, J. F., Barcak, G. J., Chandler, M. S., Redfield, R. J., and Smith, H. O. (1989) J. Bacteriol. 171, 3796-3802
19. Wittlin, S., Rosel, J., Hofmann, F., and Stover, D. R. (1999) Eur. J. Biochem. 265, 384-393
20. Willson, P. J., Albritton, W. L., Slaney, L., and Setlow, J. K. (1989) Antimicrob. Agents Chemother. 33, 1627-1630
21. Anderson, P., Johnston, R. B., Jr., and Smith, D. H. (1972) J. Clin. Invest. 51, 31-38
22. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
23. Herriott, R. M., Meyer, E. M., and Vogt, M. (1970) J. Bacteriol. 101, 517-524
24. Carlone, G. M., Thomas, M. L., Rumschlag, H. S., and Sottnek, F. O. (1986) J. Clin. Microbiol. 24, 330-332
25. Laemmli, U. K. (1970) Nature 227, 680-685
26. Cope, L. D., Pelzel, S. E., Latimer, J. L., and Hansen, E. J. (1990) Infect. Immun. 58, 3312-3318
27. Setlow, J. K., Boling, M. E., Allison, D. P., and Beattie, K. L. (1973) J. Bacteriol. 115, 153-161
28. Matsudaira, P. (1987) J. Biol. Chem. 262, 10035-10038
29. Barrett, A. J., and Rawlings, N. D. (1995) Arch. Biochem. Biophys. 318, 247-250
30. Rao, M. B., Tanksale, A. M., Ghatge, M. S., and Deshpande, V. V. (1998) Microbiol. Mol. Biol. Rev. 62, 597-635
31. Rawlings, N. D., and Barrett, A. J. (1993) Biochem. J. 290, 205-218
32. Ohnishi, Y., Beppu, T., and Horinouchi, S. (1997) J. Biochem. (Tokyo) 121, 902-913
33. Lo, R. Y., Strathdee, C. A., Shewen, P. E., and Cooney, B. J. (1991) Infect. Immun. 59, 3398-3406
34. Krem, M. M., and Di Cera, E. (2001) EMBO J. 20, 3036-3045
35. Gomis-Ruth, F. X., Gomez, M., Bode, W., Huber, R., and Aviles, F. X. (1995) EMBO J. 14, 4387-4394
36. Read, R. J., and James, M. N. (1988) J. Mol. Biol. 200, 523-551
37. Benjelloun-Touimi, Z., Tahar, M. S., Montecucco, C., Sansonetti, P. J., and Parsot, C. (1998) Microbiology 144, 1815-1822
38. Plaut, A. G., and Bachovchin, W. W. (1994) Methods Enzymol. 244, 137-151
39. Kasche, V., Lummer, K., Nurk, A., Piotraschke, E., Rieks, A., Stoeva, S., and Voelter, W. (1999) Biochim. Biophys. Acta 1433, 76-86
40. Michetti, M., Salamino, F., Tedesco, I., Averna, M., Minafra, R., Melloni, E., and Pontremoli, S. (1996) FEBS Lett. 392, 11-15
41. Weinheimer, S. P., McCann, P. J., 3rd, O'Boyle, D. R., 2nd, Stevens, J. T., Boyd, B. A., Drier, D. A., Yamanaka, G. A., DiIanni, C. L., Deckman, I. C., and Cordingley, M. G. (1993) J. Virol. 67, 5813-5822
42. Guan, C., Cui, T., Rao, V., Liao, W., Benner, J., Lin, C. L., and Comb, D. (1996) J. Biol. Chem. 271, 1732-1737