Uncharged thioflavin-T derivatives bind to amyloid-beta protein with high affinity and readily enter the brain

Life Sciences

Volumn 69, Issue 13, 2001, Pages 1471-1484

  Klunk, William E ^a,b   Wang, Yanming ^a   Huang, Guo feng ^a   Debnath, Manik L ^a   Holt, Daniel P ^a   Mathis, Chester A ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

Alzheimer's disease; Amyloid beta protein; Benzothiazole; Carbon 11; Neurofibrillary tangles; Positron emission tomography; Thioflavin T

Indexed keywords

2 (4' AMINOPHENYL) 6 METHYLBENZOTHIAZOLE; 2 [4' (DIMETHYLAMINO)PHENYL] 6 METHYLBENZOTHIAZOLE; 2 [4' (METHYLAMINO)PHENYL] 6 METHYLBENZOTHIAZOLE; AMYLOID BETA PROTEIN; BENZOTHIAZOLE DERIVATIVE; CARBON 11; THIOFLAVINE; UNCLASSIFIED DRUG;

ALZHEIMER DISEASE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BETA SHEET; BINDING AFFINITY; BRAIN TISSUE; CADAVER; CONTROLLED STUDY; DISEASE MARKER; DRUG CLEARANCE; DRUG SCREENING; DRUG STRUCTURE; DRUG SYNTHESIS; FEMALE; HUMAN; HUMAN TISSUE; IN VIVO STUDY; LIPOPHILICITY; MOUSE; NEUROFIBRILLARY TANGLE; NONHUMAN; PH; POSITRON EMISSION TOMOGRAPHY; PROTEIN BINDING; RADIOACTIVITY; STAINING;

ALZHEIMER DISEASE; AMYLOID BETA-PROTEIN; ANIMALS; BRAIN; CHEMISTRY, PHYSICAL; FEMALE; FLUORESCENT DYES; HISTOCYTOCHEMISTRY; HYDROGEN-ION CONCENTRATION; MICE; PROTEIN BINDING; SOLUBILITY; SPECTROMETRY, FLUORESCENCE; THIAZOLES; TOMOGRAPHY, EMISSION-COMPUTED;

RODENTIA;

EID: 0035902875     PISSN: 00243205     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0024-3205(01)01232-2     Document Type: Article

References (28)

1. Consensus report of the Working Group on: "Molecular and Biochemical Markers of Alz-heimer's Disease"
2. Biomarkers of Alzheimer disease
3. Biological markers of Alzheimer's disease
4. Secretase inhibitors as therapeutics for Alzheimer's disease
5. Cellular mechanisms of beta-amyloid production and secretion
6. Beta-secretase revealed: Starting gate for race to novel therapies for Alzheimer's disease
7. Immunization with amyloid-beta attenuates Alzheimer-disease-like pathology in the PDAPP mouse
8. Development of small molecule probes for the beta-amyloid protein of Alzheimer's disease
9. Chrysamine G binding to Alzheimer and control brain: Autopsy study of a new amyloid probe
10. X-34, a fluorescent derivative of Congo red: A novel histochemical stain for Alzheimer's disease pathology
11. Synthesis of a lipophilic radioiodinated ligand with high affinity to amyloid protein in Alzheimer's disease brain tissue
12. In vivo detection of amyloid plaques in a mouse model of Alzheimer's disease
13. An improved thioflavine S method for staining neurofibrillary tangles and senile plaques in Alzheimer's disease
14. A comparative study of histological and immunohistochemical methods for neurofibrillary tangles and senile plaques in Alzheimer's disease
15. Quantification of beta-sheet amyloid fibril structures with thioflavin T
16. On the histochemistry of azo group-free thiazole dyes
17. Selectively 2H-labeled Glu/Asp: Application to pKa measurements in Abeta amyloid peptides
18. An improved synthesis of (3-N-[11C]methyl)spiperone
19. Quantifying amyloid beta-peptide (Abeta) aggregation using the Congo red-Abeta (CR-abeta) spectrophotometric assay
20. Methods in binding studies
21. Gas phase basicities of 1,3-benzazoles: Benzimidazole, benzox-azole, benzothiazole, benzoselenazole and benzotellurazole
22. Thioflavin T interaction with amyloid β-sheet structures
23. X-ray diffraction from intraneuronal paired helical filaments and extraneuronal amyloid fibers in Alzheimer disease indicates cross-beta conformation
24. Fluorine-18-altanserin: A radioligand for the study of serotonin receptors with PET: Radiolabeling and in vivo biologic behavior in rats
25. In vivo labeling of cocaine binding sites on dopamine transporters with [3H]WIN 35,428
26. Quantitation of carbon-11-labeled raclopride in rat striatum using positron emission tomography
27. [11C](1)McN5652 as a radiotracer for imaging serotonin uptake sites with PET
28. [11C]WAY 100635: A radioligand for imaging 5-HT1A receptors with positron emission tomography