An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast

Journal of Cell Biology

Volumn 153, Issue 2, 2001, Pages 307-318

  Long, Roy M ^a   Gu, Wei ^b   Meng, Xiuhua ^b   Gonsalvez, Graydon ^a   Singer, Robert H ^b   Chartrand, Pascal ^b  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

ASH1; Nuclear RNA binding protein; RNA localization; Three hybrid; Yeast

Indexed keywords

MESSENGER RNA; NUCLEAR RNA; NUCLEAR RNA BINDING PROTEIN; PROTEIN; PROTEIN ASH1P; PROTEIN LOC1P; RNA BINDING PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; ASH1 GENE; FUNGAL GENE; LOC1 GENE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN RNA BINDING; RNA ANALYSIS; SACCHAROMYCES CEREVISIAE; UNTRANSLATED REGION; YEAST;

3' UNTRANSLATED REGIONS; AMINO ACID SEQUENCE; CELL NUCLEUS; DNA-BINDING PROTEINS; FUNGAL PROTEINS; GENES, REPORTER; IN SITU HYBRIDIZATION, FLUORESCENCE; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PLASMIDS; PRECIPITIN TESTS; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; REGULATORY SEQUENCES, NUCLEIC ACID; REPRESSOR PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS; TWO-HYBRID SYSTEM TECHNIQUES;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 0035897422     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.153.2.307     Document Type: Article

References (44)

1. Localization and anchoring of mRNA in budding yeast
2. Localization of ASH1 mRNA particles in living yeast
3. Asymmetric accumulation of Ash1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells
4. She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p
5. Structural elements required for the localization of ASH1 mRNA and of a green fluorescent protein reporter particle in vivo
6. A Xenopus protein related to hnRNPI has a role in cytoplasmic RNA localization
7. Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast
8. A novel nuclear pore protein Nup133p with distinct roles in poly(A)+RNA transport and nuclear pore distribution
9. Bni1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis
10. Staufen protein associates with the 3′UTR of bicoid mRNA to form particles that moves in a microtubule-dependent manner
11. A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm
12. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites
13. ASH1 mRNA localization in yeast involves multiple secondary structural elements and Ash1 protein translation
14. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes
15. Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast
16. A new efficient gene disruption cassette for repeated use in budding yeast
17. Identification of MYO4, a second class V myosin gene in yeast
18. hnRNPA2 selectively binds the cytoplasmic transport sequence of myelin basic protein mRNA
19. Mother cell-specific HO expression in budding yeast depends on the unconventional myosin Myo4p and other cytoplasmic proteins
20. Regulating the fate of mRNA: The control of cellular iron metabolism
21. Bni1p implicated in cytoskeletal control is a putative target of Rholp small GTP binding protein in Saccharomyces cerevisiae
22. Squid hnRNP protein promotes apical cytoplasmic transport and localisation of Drosophila pair-rule transcripts
23. A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export
24. Specific association between the human DNA repair proteins XPA and ERCC1
25. Spatial consequences of defective processing of specific yeast mRNAs revealed by fluorescent in situ hybridization
26. Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA
27. She2p is a novel RNA-binding protein that recruits the Myo4p/She3p complex to ASH1 mRNA
28. Ash1p is a site-specific DNA-binding protein that actively represses transcription
29. Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins
30. Regulating the HO endonuclease in yeast
31. Specific isoforms of Squid, a Drosophila hnRNp, perform distinct roles in Gurken localization during oogenesis
32. Characterization of a beta-actin mRNA zipcode-binding protein
33. Reconstitution of nuclear protein transport with semi-intact yeast cells
34. A three-hybrid system to detect RNA-protein interactions in vivo
35. Identification of asymmetrically localized determinant, Ash1p, required for lineage-specific transcription of the yeast HO gene
36. Staufen, a gene required to localize maternal RNAs in the Drosophila egg
37. A conserved double-stranded RNA binding domain
38. Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast
39. The myosin motor, Myo4p, binds ASH1 mRNA via the adapter protein, She3p
40. A novel fluorescence-activated cell sorter-based screen for yeast endocytosin mutants identifies a yeast homologue of mammalian eps15
41. Genetic analysis of the bipolar pattern of bud site selection in the yeast Saccharomyces cerevisiae
42. A conserved RNA-binding protein that regulates sexual fates in the C. elegans hermaphrodite germ line
43. Random mutagenesis of genesized DNA molecules by use of PCR with Taq DNA polymerase