Neuronal nitric oxide synthase generates superoxide from the oxygenase domain

Biochemical Journal

Volumn 360, Issue 1, 2001, Pages 247-253

  Yoneyama, H ^a   Yamamoto, A ^a   Kosaka, H ^a  

^a KAGAWA UNIVERSITY   (Japan)

Author keywords

Adrenochrome; C415A nNOS; DMPO; ESR spin trap; Reductase

Indexed keywords

BIOASSAY; MIXTURES; NITROGEN OXIDES; PARAMAGNETIC RESONANCE;

SPIN TRAP;

ENZYMES;

5,5 DIMETHYL 1 PYRROLINE 1 OXIDE; ADRENOCHROME; ARGININE; FLAVOPROTEIN; NITRIC OXIDE SYNTHASE; OXIDOREDUCTASE; OXYGENASE; SODIUM CYANATE; SUPEROXIDE;

ARTICLE; CATALYSIS; ELECTRON SPIN RESONANCE; ENZYME ACTIVITY; MOUSE; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; SIGNAL TRANSDUCTION;

ANIMALS; ARGININE; CATALYSIS; DNA, COMPLEMENTARY; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTRON SPIN RESONANCE SPECTROSCOPY; HEME; MICE; MUTATION; NEURONS; NITRIC OXIDE SYNTHASE; OXIDATION-REDUCTION; OXYGENASES; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SODIUM CYANIDE; SPIN TRAPPING; SUPEROXIDES; TIME FACTORS;

EID: 0035890621     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3600247     Document Type: Article

References (35)

1. Nitric oxide synthases: Roles, tolls, and controls
2. Reaction of neuronal nitric oxide synthase with the nitric oxide spin-trapping agent, iron complexed with N-dithiocarboxysarcosine
3. Neuronal nitric oxide synthase, a modular enzyme formed by convergent evolution: Structure studies of a cysteine thiolate-liganded heine protein that hydroxylates L-arginine to produce NO as a cellular signal
4. Generation of superoxide by purified brain nitric oxide synthase
5. Superoxide generation by endothelial nitric oxide synthase: The influence of cofactors
6. Inducible nitric-oxide synthase generates superoxide from the reductase domain
7. Superoxide and peroxynitrite generation from inducible nitric oxide synthase in macrophages
8. Peroxynitrite-mediated attenuation of alpha- and beta-adrenoceptor agonist-induced vascular responses in vivo
9. 2+/calmodulin-dependent and tetrahydrobiopterin regulatory process
10. Crystal structure of constitutive endothelial nitric oxide synthase: A paradigm for pterin function involving a novel metal center
11. Upregulation of neuronal nitric oxide synthase and mRNA, and selective sparing of nitric oxide synthase-containing neurons after focal cerebral ischemia in rat
12. Generation of nitric oxide and superoxide during reperfusion after focal cerebral ischemia in rats
13. Involvement of the reductase domain of neuronal nitric oxide synthase in superoxide anion production
14. Mechanism of superoxide generation by neuronal nitric-oxide synthase
15. Does nitric oxide synthase catalyze the synthesis of superoxide?
16. A key negative control experiment provides evidence that nitric oxide synthase does not catalyze superoxide formation
17. Characterization of C415 mutants of neuronal nitric oxide synthase
18. Structural diversity of neuronal nitric oxide synthase mRNA in the nervous system
19. In vivo analysis of integration of membrane proteins in Escherichia coli
20. Molecular cloning of sheep and goat ferredoxin reductase messenger ribonucleic acids, and identification of an alternatively spliced form of sheep ferredoxin reductase
21. Calmodulins from muscles of marine invertebrates, scallop end sea anemone
22. High-level expression of functional rat neuronal nitric oxide synthase in Escherichia coli
23. Large-scale purification of rat brain nitric oxide synthase from Bacutovirus overexpression system
24. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
25. Simultaneous determination of hemes a, b, and c from pyridine hemochrome spectra
26. Determination of nitric oxide synthase cofactors
27. Rapid assay for nitric oxide synthase using thin-layer chromatography
28. Characterization of the flavoprotein moieties of NADPH-sulfite reductase from Salmonella typhimurium and Escherichia coli. Physicochemical and catalytic properties, amino acid sequence deduced from DNA sequence of cysJ, and comparison with NADPH-cytochrome P-450 reductase
29. Cloned, expressed rat cerebellar nitric oxide synthase contains stoichiometric amounts of heme, which binds carbon monoxide
30. Tetrahydrobiopterin-dependent inhibition of superoxide generation from neuronal nitric oxide synthase
31. Tetrahydrobiopterin-free neuronal nitric oxide synthase: Evidence for two identical highly anticooperative pteridine binding sites
32. The C331A mutant of neuronal nitric-oxide synthase is defective in arginine binding
33. An autoinhibitory control element defines calcium-regulated isoforms of nitric oxide synthase
34. L-Arginine improves endothelial function in renal artery of hypertensive Dahl rats
35. Nitric oxide in the pathogenesis of vascular disease