Early visual changes in reflected light on non-stained brain sections after focal ischemia mirror the area of ischemic damage

Journal of Neuroscience Methods

Volumn 111, Issue 1, 2001, Pages 67-73

  Kharlamov, A ^a   Kirn, O K ^a   Jones, S C ^a  

^a ALLEGHENY GENERAL HOSPITAL   (United States)

Author keywords

Brain shrinkage; Focal ischemia; Immunostained sections; Ischemic region; Measurement; Microtubule associated protein; Microtubule associated protein 2 antibody; Non stained sections; Reflective change

Indexed keywords

COLORING AGENT; DIAGNOSTIC AGENT; MICROTUBULE ASSOCIATED PROTEIN; PHENOTHIAZINE DERIVATIVE; THIONINE;

ANIMAL; ARTICLE; BRAIN; BRAIN ISCHEMIA; IMAGE ENHANCEMENT; IMMUNOHISTOCHEMISTRY; INSTRUMENTATION; LIGHT; MALE; METABOLISM; METHODOLOGY; MICROSCOPY; MICROTOMY; NERVE DEGENERATION; PATHOLOGY; PATHOPHYSIOLOGY; RAT; SPRAGUE DAWLEY RAT; TIME;

ANIMALS; BRAIN; BRAIN ISCHEMIA; COLORING AGENTS; IMAGE ENHANCEMENT; IMMUNOHISTOCHEMISTRY; LIGHT; MALE; MICROSCOPY; MICROTOMY; MICROTUBULE-ASSOCIATED PROTEINS; NERVE DEGENERATION; PHENOTHIAZINES; RATS; RATS, SPRAGUE-DAWLEY; TIME FACTORS;

EID: 0035888929     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(01)00444-7     Document Type: Article

References (20)

1. Avendaño C, Roda JM, Carceller F, Díez-Tejedor E. Morphometric study of focal cerebral ischemia in rats: a stereological evaluation. Brain Res 1995;673:83-92.
2. Bederson JB, Pitts LH, Germano SM, Nishimura MC, Davis RL, Bartkowski HM. Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats. Stroke 1986;17:1304-8.
3. Bednar MM, Fanburg JC, Anderson ML, Raymond SJ, Dooley RH, Gross CE. Comparison of triphenyltetrazolium dye with light microscopic evaluation in a rabbit model of acute cerebral ischaemia. Neurol Res 1994;16:129-32.
4. Bland JM, Altman DG. Measuring agreement in method comparison studies. Stat Meth Med Res 1999;8:135-60.
5. Bose B, Jones SC, Lorig R, Friel HT, Weinstein M, Little JR. Evolving focal cerebral ischemia in cats: spatial correlation of nuclear magnetic resonance imaging, cerebral blood flow, tetrazolium staining, and histopathology. Stroke 1988;19:28-37.
6. Bose B, Osterholm JL, Berry R. A reproducible experimental model of focal cerebral ischemia in the cat. Brain Res 1984;311:385-91.
7. Callaway JK, Knight MJ, Watkins DJ, Beart PM, Jarrott B, Delaney PM. A novel, rapid, computerised method for quantitation of neuronal damage in a rat model of stroke. J Neurosci Meth 2000;102:53-60.
8. Chen ST, Hsu CY, Hogan EL, Maricq H, Balentine JD. A model of focal ischemic stroke in the rat: reproducible extensive cortical infarction. Stroke 1986;17:738-43.
9. Dawson DA, Hallenbeck JM. Acute focal ischemia-induced alterations in MAP2 immunostaining: description of temporal changes and utilization as a marker for volumetric assessment of acute brain injury. J Cereb Blood Flow Metab 1996;16:170-4.
10. Garcia JH, Mitchem HL, Briggs L, Morawetz R, Hudetz AG, Hazelrig JB, et al. Transient focal ischemia in subhuman primates: neuronal injury as a function of local cerebral blood flow. J Neuropathol Exp Neurol 1983;42:44-60.
11. Garcia JH, Yoshida Y, Chen H, Li Y, Zhang ZG, Lian J, et al. Progression from ischemic injury to infarct following middle cerebral artery occlusion in the rat. Am J Pathol 1993;142:623-35.
12. Halpain S, Greengard P. Activation of NMDA receptors induces rapid dephosphorylation of the cytoskeletal protein MAP2. Neuron 1990;5:237-46.
13. Hatfield RH, Mendelow AD, Perry RH, Alvarez LM, Modha P. Triphenyltetrazolium chloride (TTC) as a marker for ischaemic changes in rat brain following permanent middle cerebral artery occlusion. Neuropathol Appl Neurobiol 1991;17:61-7.
14. Hsu SM, Raine L. Protein A, avidin, and biotin in immunohisto-chemistry. J Histochem Cytochem 1981;29:1349-53.
15. Hu W, Kharlamov A, Wang Y, Perez-Trepichio AD, Jones SC. Directed sampling for electrolyte analysis and water content of micro-punch samples shows large differences between normal and ischemic rat brain cortex. Brain Res 2000;868:370-5.
16. 2 reactivity and heterogeneity of cerebral blood flow in ischemic, border zone, and normal cortex. Am J Physiol Heart Circ Physiol 1989;257:H473-82.
17. Klatzo I. Neuropathological aspects of brain edema. J Neuropathol Exp Neurol 1967;26:1-14.
18. Lin T-N, He YY, Wu G, Khan M, Hsu CY. Effect of brain edema on infarct volume in a focal cerebral ischemia model in rats. Stroke 1993;24:117-21.
19. Liszczak TM, Hedley-Whyte ET, Adams JF, Han DH, Kolluri VS, Vacanti FX, et al. Limitations of tetrazolium salts in delineating infarcted brain. Acta Neuropathol 1984;65:150-7.
20. Little JR, Awad I. Pathophysiology of focal cerebral ischemia. In: Smith RR, editor. Stroke and the extracranial vessels. New York: Raven Press, 1984:39-46.