A dot-blot-immunoassay for semen identification using a polyclonal antibody against semenogelin, a powerful seminal marker

Forensic Science International

Volumn 122, Issue 1, 2001, Pages 27-34

  Sato, Itaru ^a,b   Yoshiike, Miki ^b   Yamasaki, Tadashi ^c   Yoshida, Kaoru ^b   Takano, Shoichi ^c   Mukai, Toshiji ^b   Iwamoto, Teruaki ^b  

^a Kanagawa Prefectural Police   (Japan)

^b ST MARIANNA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c BML   (Japan)

Author keywords

Forensic science; Immunoassay; Semen; Semenogelin

Indexed keywords

BIOLOGICAL MARKER; POLYCLONAL ANTIBODY; POLYPEPTIDE; PROTEIN; PROTEIN SEMENOGELIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ADULT; ANTIBODY PRODUCTION; ANTIGEN DETECTION; ANTIGENICITY; ARTICLE; BACULOVIRUS; BODY FLUID; CONTROLLED STUDY; DILUTION; FEMALE; FILTER; HUMAN; IMMUNOBLOTTING; IMMUNOREACTIVITY; MALE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; PROTEIN STABILITY; RABBIT; RAPE; SEMEN ANALYSIS; SEMINAL PLASMA; SERUM; TEMPERATURE;

EID: 0035888364     PISSN: 03790738     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0379-0738(01)00435-2     Document Type: Article

References (30)

1. Isolation and characterization of a semen-specific protein from human seminal plasma: A potential new marker for semen identification
2. Detection of prostate-specific antigen by ELISA
3. Prostate-specific antigen: A critical assessment of the most useful tumor marker for adenocarcinoma of the prostate
4. Evaluation of prostate-specific antigen (PSA) membrane test assays for the forensic identification of seminal fluid
5. Characterization of a monoclonal antibody to a conserved epitope on human seminal vesicle-specific peptides: A novel probe/marker system for semen identification
6. An enzyme-linked immunosorbent assay (ELISA) for human semen identification based on a biotinylated monoclonal antibody to a seminal vesicle-specific antigen
7. Evaluation of MHS-5 in detecting seminal fluid in vaginal swabs
8. Semenogelin I: A coagulum forming, multifunctional seminal vesicle protein
9. Isolation and characterization of the major gel proteins in human semen, semenogelin I and semenogelin II
10. Molecular cloning of epididymal and seminal vesicular transcripts encoding a semenogelin-related protein
11. Purification and characterization of a sperm motility inhibitor in human seminal plasma
12. Human seminal vesicle-specific antigen is a substrate-specific antigen (or p30)
13. Gene structure of semenogelin I and II
14. Characterization of recombinant precursor proteins of the human seminal plasma sperm motility inhibitor synthesized in insect cells
15. A sandwich enzyme-linked immunosorbent assay for ABO blood typing of semen using anti-p84 monoclonal antibody as a marker of blood group substance in semen
16. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
17. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
18. Transferring subtyping of blood stains and semen using isoelectric focusing and immunoblotting
19. Deoxyribonuclease 1 (DNase 1) typing from semen stains: Low enzyme activity in vaginal fluids does not interfere with seminal DNase 1 typing from mixture stains
20. HUTTH01 validation studies: Effect of substrate, environment, and mixtures
21. Multiplexing of Y chromosome-specific STRs and performance for mixed samples
22. Male DNA typing from 25-year-old vaginal swabs using Y chromosomal STR polymorphisms in a retrial request case
23. DNA typing of azoospermic semen at the D1S80 locus
24. Interaction between seminal plasma and spermatozoa
25. Surgery of the seminal vesicles
26. Sperm motility inhibitor from human seminal plasma: Presence of a precursor molecule in seminal vesicle fluid and its molecular processing after ejaculation
27. Sperm motility inhibitor from human seminal plasma: Association with semen coagulum
28. Immunohistochemical localization of the MHS-5 antigen in principal cells of human seminal vesicle epithelium
29. Characterization of prostate-specific antigen proteolytic activity on its major physiological substrate, the sperm motility inhibitor precursor/semenogelin I
30. Semenogelin, the predominant protein in human semen