A method to measure the interaction of Rac/Cdc42 with their binding partners using fluorescence resonance energy transfer between mutants of green fluorescent protein

Analytical Biochemistry

Volumn 296, Issue 2, 2001, Pages 208-217

  Graham, Debbie L ^a   Lowe, Peter N ^a   Chalk, Peter A ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Cdc42; Fluorescence resonance energy transfer; Green fluorescent protein; Isothermal titration calorimetry; P21 activated kinase; Rac; Scintillation proximity assay

Indexed keywords

AMINO ACIDS; BIOSENSORS; ENERGY TRANSFER; ENZYMES; FORSTER RESONANCE ENERGY TRANSFER; RESONANCE;

CDC42; FLUORESCENCE RESONANCE ENERGY TRANSFER; GREEN FLUORESCENT PROTEIN; ISOTHERMAL TITRATION CALORIMETRY; P-21 ACTIVATED KINASE;

BINDING ENERGY;

GREEN FLUORESCENT PROTEIN; MUTANT PROTEIN; PHOSPHOTRANSFERASE; PROTEIN CDC42; RAC PROTEIN;

ARTICLE; BINDING AFFINITY; BIOSENSOR; CELL COMPARTMENTALIZATION; ENERGY TRANSFER; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN LOCALIZATION;

EID: 0035884384     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5306     Document Type: Article

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