Mechanistic aspects of DnaA-RepA interaction as revealed by yeast forward and reverse two-hybrid analysis

EMBO Journal

Volumn 20, Issue 16, 2001, Pages 4577-4587

  Sharma, R ^a   Kachroo, A ^a   Bastia, D ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Helicase loading; Ori unwinding; Protein protein interaction; Replication initiation; Yeast reverse two hybrid

Indexed keywords

HELICASE; REPLICATION PROTEIN A;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; COVALENT BOND; DNA BINDING; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; YEAST;

ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEINS; BINDING SITES; CHROMATOGRAPHY, AFFINITY; DNA HELICASES; DNA REPLICATION; DNA, BACTERIAL; DNA-BINDING PROTEINS; DNAB HELICASES; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; LEUCINE ZIPPERS; MUTAGENESIS; PLASMIDS; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; PROTEINS; REPLICATION ORIGIN; SACCHAROMYCES CEREVISIAE; TRANS-ACTIVATORS; TWO-HYBRID SYSTEM TECHNIQUES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0035881460     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.16.4577     Document Type: Article

References (36)

1. The E.coli cell cycle and the plasmid R1 replication cycle in the absence of the DnaA protein
2. DNA-induced conformational changes in bacteriophage 434 repressor
3. Replication and expression of constructed plasmid chimeras in transformed Escherichia coli - A review
4. Mechanism of recruitment of DnaB helicase to the replication origin of the plasmid pSC101
5. Replisome assembly at oriC, the replication origin of E.coli, reveals an explanation for initiation sites outside an origin
6. A novel genetic system to detect protein-protein interactions
7. Membrane regulation of the chromosomal replication activity of E.coli DnaA requires a discrete site on the protein
8. DnaA protein is required for replication of the minimal replicon of the broad-host-range plasmid RK2 in Escherichia coli
9. Host participation in plasmid maintenance: Dependence upon dnaA of replicons derived from P1 and F
10. Mutations of temperature sensitivity in R plasmid pSC101
11. Replication of plasmid pSC101 in Escherichia coli K12: Requirement for dnaA function
12. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast
13. A bacterial two-hybrid system based on a reconstituted signal transduction pathway
14. Structural and functional analysis of a replication enhancer: Separation of the enhancer activity from origin function by mutational dissection of the replication origin gamma of plasmid R6K
15. Helicase delivery and activation by DnaA and TrfA proteins during the initiation of replication of the broad host range plasmid RK2
16. Role of TrfA and DnaA proteins in origin opening during initiation of DNA replication of the broad host range plasmid RK2
17. Cryptic single-stranded-DNA binding activities of the phage lambda P and Escherichia coli DnaC replication initiation proteins facilitate the transfer of E.coli DnaB helicase onto DNA
18. Mechanistic studies of initiator-initiator interaction and replication initiation
19. The replication of plasmid pSC101
20. DnaA protein directs the binding of DnaB protein in initiation of DNA replication in Escherichia coli
21. Functions of the DnaA protein of Escherichia coli in replication and transcription
22. Functional domains of DnaA proteins
23. The partition (par) locus of pSC101 is an enhancer of plasmid incompatibility
24. Activation of distant replication origins in vivo by DNA looping as revealed by a novel mutant form of an initiator protein defective in cooperativity at a distance
25. Spacing requirements between LexA operator half-sites can be relaxed by fusing the LexA DNA binding domain with some alternative dimerization domains
26. High copy number plasmids compatible with commonly used cloning vectors
27. The interaction domains of the DnaA and DnaB replication proteins of Escherichia coli
28. The central lysine in the P-loop motif of the Escherichia coli DnaA protein is essential for initiating DNA replication from the chromosomal origin, oriC, and the F factor origin, oriS, but is dispensable for initiation from the P1 plasmid origin, oriR
29. Mechanism of origin unwinding: Sequential binding of DnaA to double- and single-stranded DNA
30. The integration host factor of Escherichia coli binds to bent DNA at the origin of replication of the plasmid pSC101
31. Cooperativity at a distance promoted by the combined action of two replication initiator proteins and a DNA bending protein at the replication origin of pSC101
32. Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC
33. The Escherichia coli dnaA gene: Four functional domains
34. Structural and functional analysis of the par region of the pSC101 plasmid
35. Primary structure of the essential replicon of the plasmid pSC101
36. DNA-protein interaction at the origin of DNA replication of the plasmid pSC101