Photocytotoxicity of lipofuscin in human retinal pigment epithelial cells

Free Radical Biology and Medicine

Volumn 31, Issue 2, 2001, Pages 256-265

  Davies, Sallyanne ^a   Elliott, Michael H ^b   Floor, Eric ^b   Truscott, T George ^c   Zareba, Mariusz ^d   Sarna, Tadeusz ^d   Shamsi, Farrukh A ^a   Boulton, Michael E ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF KANSAS   (United States)

^c KEELE UNIVERSITY   (United Kingdom)

^d JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

A2E; Free radicals; Light damage; Lipofuscin; Lysosomes; Oxidation; Retinal pigment epithelium

Indexed keywords

4 HYDROXYNONENAL; CARBONYL DERIVATIVE; LIPOFUSCIN; M PROTEIN; MALONALDEHYDE;

AGING; ARTICLE; CELL CULTURE; CELL DEATH; CELL STRUCTURE; CELL VACUOLE; CELL VIABILITY; CONTROLLED STUDY; CYTOPLASM; CYTOTOXICITY; DARKNESS; EPITHELIUM CELL; HUMAN; HUMAN CELL; LIGHT; LIPID PEROXIDATION; LYSOSOME; MEMBRANE; OXIDATION; PHOTOTOXICITY; PIGMENT EPITHELIUM; PRIORITY JOURNAL; RETINA DISEASE; TEMPERATURE;

AGING; ANTIOXIDANTS; CELL SURVIVAL; CELLS, CULTURED; EPITHELIAL CELLS; FREE RADICALS; HUMANS; LIPID PEROXIDATION; LIPOFUSCIN; LYSOSOMES; PHOTOBIOLOGY; PIGMENT EPITHELIUM OF EYE;

EID: 0035879852     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(01)00582-2     Document Type: Article

References (51)

1. Aging human RPE: Morphometric analysis of macular, equatorial, and peripheral cells
2. The fate of the phagosome: Conversion to 'age pigment' and impact in human retinal pigment epithelium
3. The formation of autofluorescent granules in cultured human RPE following longterm challenge with rod outer segments
4. Retinal age pigments generated by self-assembling lysosomotropic detergents
5. Biochemical basis of lipofuscin, ceroid, and age pigment-like fluorophores
6. Lipofuscin is a photoinducible free radical generator
7. Blue light-induced reactivity of retinal age pigment
8. Blue light-induced singlet oxygen generation by retinal lipofuscin in non-polar media
9. Photoreactivity of the retinal age pigment lipofuscin
10. Photodamage to human RPE cells by A2E, a retinoid component of lipofuscin
11. The nature of retinal radiation damage: Dependence on wavelength, power level and exposure time
12. Retinitis pigmentosa: A preliminary report on tissue culture studies of retinal pigment epithelial cells from 8 affected human eyes
13. Age-related changes in the morphology, absorption and fluorescence of melanosomes and lipofuscin granules of the retinal pigment epithelium
14. Repigmentation of human retinal pigment epithelial cells in vitro
15. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
16. Exposure of cells to nonlethal concentrations of hydrogen peroxide induces degeneration-repair mechanisms involving lysosomal destabilization
17. Excess brain protein oxidation and enzyme dysfunction in normal aging and in Alzheimer disease
18. Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal
19. Increased protein oxidation in human substantia nigra pars compacta in comparison with basal ganglia and prefrontal cortex measured with an improved dinitrophenylhydrazine assay
20. A rapid, sensitive, and specific method for the determination of protein in dilute solution
21. Modification of protein surface hydrophobicity and methionine oxidation by oxidative systems
22. Determination of protein carbonyl groups by immunoblotting
23. Reactive oxygen-mediated protein oxidation in aging and disease
24. Oxidative damage and age-related macular degeneration
25. 2 induce catalase and metallothionein gene expression in human retinal pigment epithelial cells
26. Induction of glutathione S-transferase hGST 5.8 is an early response to oxidative stress in RPE cells
27. Evaluation of oxidative processes in human pigment epithelial cells associated with retinal outer segment phagocytosis
28. The photochemistry of human retinal lipofuscin as studied by EPR
29. Susceptibility of glutathione peroxidase and glutathione reductase to oxidative damage and the protective effect of spin trapping agents
30. Potential mechanisms of photodynamic inactivation of virus by methylene blue. I. RNA-protein crosslinks and other oxidative lesions in Q beta bacteriophage
31. Protein hydroperoxides and carbonyl groups generated by porphyrin-induced photo-oxidation of bovine serum albumin
32. The reaction of singlet oxygen with proteins, with special reference to crystallins
33. Role of free radicals and catalytic metal ions in human disease: An overview
34. Metal ion-catalyzed oxidation of proteins: Biochemical mechanism and biological consequences
35. Chemical characterization of a protein-4-hydroxy-2-nonenal cross-link: Immunochemical detection in mitochondria exposed to oxidative stress
36. Protein-bound acrolein: A novel marker of oxidative stress in Alzheimer's disease
37. Free radical damage to proteins: The influence of the relative localization of radical generation, antioxidants, and target proteins
38. Mitochondria generate superoxide anion radicals and hydrogen peroxide
39. Photo-oxidative disruption of lysosomal membranes causes apoptosis of cultured human fibroblasts
40. Lipofuscin accumulation in cultured retinal pigment epithelial cells causes enhanced sensitivity to blue light irradiation
41. Biochemistry and pathology of radical-mediated protein oxidation
42. Hydrogen peroxide inhibits the vacuolar H+-ATPase in brain synaptic vesicles at micromolar concentrations
43. Mutations in the CYS4 gene provide evidence for regulation of the yeast vacuolar H+-ATPase by oxidation and reduction in vivo
44. Acidification of phagosomes and degradation of rod outer segments in rat retinal pigment epithelium
45. Isolation and one-step preparation of A2E and iso-A2E, fluorophores from human retinal pigment epithelium
46. Lipofuscin and other storage deposits in the RPE
47. Inhibition of lysosomal degradative functions in RPE cells by a retinoid component of lipofuscin
48. The lipofuscin fluorophore A2E mediates blue light-induced damage to retinal pigmented epithelial cells
49. A2E, a lipofuscin fluorophore, in human retinal pigmented epithelial cells in culture