Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro

Theriogenology

Volumn 56, Issue 2, 2001, Pages 211-223

  Peknicova, J ^a   Capkova, J ^a   Geussova, G ^a   Ivanova, M ^b   Mollova, M ^b  

^a INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

^b BULGARIAN ACADEMY OF SCIENCES   (Bulgaria)

Author keywords

Acrosin; Intra acrosomal proteins; Secondary sperm to zona pellucida binding

Indexed keywords

ACROSIN; MONOCLONAL ANTIBODY; PROTEIN DERIVATIVE;

ACROSOME; ANIMAL; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; FEMALE; FERTILITY; FERTILIZATION; GAMETE; IN VITRO STUDY; MALE; NONHUMAN; OOCYTE; SPERM; SPERMATOZOON CAPACITATION; SWINE; ZONA PELLUCIDA;

ACROSIN; ACROSOME; ANIMALS; ANTIBODIES, MONOCLONAL; FEMALE; MALE; SPERM-OVUM INTERACTIONS; SWINE;

EID: 0035878793     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0093-691X(01)00557-X     Document Type: Article

References (30)

1. Mammalian fertilization misread? Sperm penetration of the eutheriaan zona pellucida is unlikely to be a lytic event
2. Evaluation of assay conditions for the zona-free hamster ova bioassay of boar sperm fertility
3. Autoradiographic visualization of the mouse eggs sperm receptor bound to sperm
4. Binding of boar spermatozoa to porcine oocytes: Effect of low molecular weight 17-kDa protein
5. Application of monoclonal antibodies against sperm protein s to diagnostics of human sperm pathology and for selection of suitable fertilization methods in assisted reproduction
6. Characterization of the functional domains of boar acrosin involved in nonenzymatic binding to homologous zona pellucida glycoproteins
7. Monoclonal antibodies as direct probes for human sperm acrosome reaction
8. Gamete recognition in mammals: Sperm and zona pellucida interactions
9. Characterization of monoclonal antibody to human proacrosin and its use in acrosomal status evaluation
10. Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction
11. Structure-function properties of the sperm enzyme acrosin
12. Site-directed mutagenesis of boar proacrosin reveals residues involved in binding of zona pellucida glycoproteins
13. The mammalian sperm acrosome and the acrosome reaction
14. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4
15. Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction
16. Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin
17. The polysulphate binding domain of human proacrosin/acrosin is involved in both the enzyme activation and spermatozoa-zona pellucida interaction
18. Monoclonal antibodies against boar acrosomal antigens labeling undamaged acrosomes of spermatozoa in immunofluorescence test
19. Changes in immunochemical localisation of acrosomal and sperm proteins in boar spermatozoa during capacitation and induced acrosome reaction
20. Boar proacrosin. Purification and preliminary activation studies of proacrosin isolated from ejaculated boar sperm
21. Subcellular immunochemical localization of acrosin in human spermatozoa during the acrosome reaction and zona pellucida penetration
22. Acrosin shows zona and fucose binding, novel properties for a serine proteinase
23. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
24. Biochemistry and function of acrosin
25. Characterization of boar spermadhesins by monoclonal and polyclonal antibodies and their role in binding to oocytes
26. Molecular events mediating sperm activation
27. Mammalian fertilization: Molecular aspects of gamete adhesion, exocytosis, and fusion
28. Mammalian fertilization
29. Molecular nature of a sperm acrosomal antigen recognized by HS-13 monoclonal antibody
30. Molecular characterization of an intraacrosomal antigen defined by HS-33 monoclonal antibody