Saponin permeabilization of rough microsomes from rat liver reveals a novel prothrombin pool

Biochimica et Biophysica Acta - General Subjects

Volumn 1526, Issue 3, 2001, Pages 249-256

  Tollefsen, S ^a   Wierod L ^a   Skotte, A ^a   Rob, J A ^a   Helgeland, L ^a  

^a UNIVERSITY OF OSLO   (Norway)

Author keywords

Microsome; Novel prothrombin pool; Permeabilization; Rat; Saponin

Indexed keywords

CARBON 14; POTASSIUM CHLORIDE; PROTHROMBIN; SAPONIN; SODIUM CHLORIDE; TRITON X 100; WARFARIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CARBOXYLATION; CONTROLLED STUDY; LIVER MICROSOME; MALE; MEMBRANE PERMEABILITY; MICROSOME MEMBRANE; NONHUMAN; PHASE SEPARATION; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN LOCALIZATION; PROTEIN SECRETION; PROTEIN SYNTHESIS; RAT; ROUGH ENDOPLASMIC RETICULUM; SOLUBILIZATION; TISSUE DISTRIBUTION;

ANIMALS; ANTICOAGULANTS; BUFFERS; CARBON DIOXIDE; CARBON RADIOISOTOPES; CHEMICAL FRACTIONATION; ENDOPLASMIC RETICULUM; INTRACELLULAR MEMBRANES; MALE; MICROSOMES, LIVER; PERMEABILITY; POLYETHYLENE GLYCOLS; PROTEIN PRECURSORS; PROTHROMBIN; RATS; RATS, WISTAR; SAPONINS; VITAMIN K; WARFARIN;

EID: 0035877117     PISSN: 03044165     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-4165(01)00137-4     Document Type: Article

References (32)

1. Vitamin K-dependent carboxylase
2. The submicrosomal site for the conversion of prothrombin precursor to biologically active prothrombin in rat liver
3. Vitamin K dependent carboxylase: Subcellular location of the carboxylase and enzymes involved in vitamin K metabolism in rat liver
4. Vitamin K-dependent biosynthesis of gamma-carboxyglutamic acid
5. Vitamin K dependent in vitro production of prothrombin
6. Biosynthesis and clearance of prothrombin in warfarin-treated rats
7. Prothrombin synthesis and degradation in rat hepatoma (H-35) cells: Effects of warfarin
8. Prothrombin biosynthesis: Characterization of processing events in rat liver microsomes
9. Early processing of prothrombin and factor X by the vitamin K-dependent carboxylase
10. Investigation of a possible correlation between rates of secretion and microsomal membrane association of plasma proteins synthesized by rat liver
11. Über die Strukturspezifität der Saponinhämolyse. I. Triterpensaponine und -aglykone
12. Differential permeabilization of membranes by saponin treatment of isolated rat hepatocytes. Release of secretory proteins
13. Secretion granules of the rabbit parotid. Selective removal of secretory contaminants from granule membranes
14. Assembly in vitro of a spanning membrane protein of the endoplasmic reticulum: The E1 glycoprotein of coronavirus mouse hepatitis virus A59
15. Glycosylation site binding protein, a component of oligosaccharyl transferase, is highly similar to three other 57 kd luminal proteins of the ER
16. A study of intracellular transport of secretory glycoproteins in rat liver
17. A study on the intracellular transport of prothrombin, albumin and transferrin in rat
18. Isolation of integral membrane proteins by phase partitioning with Triton X-114
19. A rapid and highly sensitive chromogenic microplate assay for quantification of rat and human prothrombin
20. Protein measurement with the Folin phenol reagent
21. Role of propeptide in vitamin K-dependent gamma-carboxylation
22. Intracellular proteolytic processing of the two-chain vitamin K-dependent coagulation factor X
23. A novel enzyme immunoassay for quantitation of rat prothrombin in microsomal subfractions
24. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
25. Lysyl hydroxylase, a collagen processing enzyme, exemplifies a novel class of luminally-oriented peripheral membrane proteins in the endoplasmic reticulum
26. Selective release of content from microsomal vesicles without membrane disassembly. I. Permeability changes induced by low detergent concentrations
27. Evidence for competition between vitamin K-dependent clotting factors for intracellular processing by the vitamin K-dependent gamma-carboxylase
28. Intracellular vesicles involved in the transport of Semliki Forest virus membrane proteins to the cell surface
29. Phase separation of integral membrane proteins in Triton X-114 solution
30. Triple helix formation of procollagen type I can occur at the rough endoplasmic reticulum membrane
31. Hydrophobic amino acids define the carboxylation recognition site in the precursor of the gamma-carboxyglutamic-acid-containing conotoxin epsilon-TxIX from the marine cone snail Conus textile