Cloning and rational mutagenesis of kexstatin I, a potent proteinaceous inhibitor of Kex2 proteinase

Biochemical Journal

Volumn 355, Issue 2, 2001, Pages 339-346

  Oda, K ^a   Oyama, H ^a   Ito, S ^a   Fukiharu, M ^a   Miyagawa, Y ^a   Takahashi, S ^a   Hirose, M ^a   Kikuchi, N ^a   Nakayama, T ^a   Shibano, Y ^a  

^a KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Furin; Inhibition; Streptomyces subtilisin inhibitors; Subtilisin

Indexed keywords

AMINO ACIDS; ENZYME INHIBITION; GENES; MUTAGENESIS; PROTEINS; SUBSTRATES;

MUTANTS;

CLONING;

ARGININE; KEXSTATIN 1; LYSINE; PROTEINASE; PROTEINASE INHIBITOR; PROTEINASE KEX2; THREONINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SPECIFICITY; GENE EXPRESSION; GENE STRUCTURE; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN SECRETION; SEQUENCE HOMOLOGY; STREPTOMYCES;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, RECOMBINANT; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROPROTEIN CONVERTASES; PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBTILISINS;

EID: 0035871221     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3550339     Document Type: Article

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