Escherichia coli flavohaemoglobin (Hmp) with equistoichiometric FAD and haem contents has a low affinity for dioxygen in the absence or presence of nitric oxide

Biochemical Journal

Volumn 353, Issue 2, 2001, Pages 207-213

  Mills, C E ^a   Sedelnikova, S ^a   So B ^a   Hughes, M N ^a   Poole, R K ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Bacterial NO detoxification; Haemoglobin; NO oxygenase

Indexed keywords

FLAVINE ADENINE NUCLEOTIDE; FLAVOHAEMOGLOBIN; FLAVOPROTEIN; NITRIC OXIDE; OXYGEN; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; DENITRIFICATION; DETOXIFICATION; ELECTRON TRANSPORT; ENZYME ACTIVITY; ESCHERICHIA COLI; HEME SYNTHESIS; HYPOXIA; OXIDATIVE STRESS; OXYGEN AFFINITY; OXYGEN CONSUMPTION; PRIORITY JOURNAL; RECEPTOR UPREGULATION; STOICHIOMETRY;

ANAEROBIOSIS; BACTERIAL PROTEINS; DIHYDROPTERIDINE REDUCTASE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLAVIN-ADENINE DINUCLEOTIDE; HEMEPROTEINS; NADH, NADPH OXIDOREDUCTASES; NITRIC OXIDE; OXYGEN; OXYGEN CONSUMPTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0035863151     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3530207     Document Type: Article

References (32)

1. New functions for the ancient globin family: Bacterial responses to nitric oxide and nitrosative stress
2. Isolation and nucleotide sequence of the hmp gone that encodes a haemoglobin-like protein in Escherichia coli K-12
3. Bacterial flavohaemoglobins: A consensus sequence and identification of a discrete enterobacterial group and of further bacterial globins
4. The haemoglobin-like protein (HMP) of Escherichia coil has ferrisiderophore reductase activity and its C-terminal domain shares homology with ferredoxin NADP+ reductases
5. Response of the NAD(P)H-oxidising flavohaemoglobin (Hmp) to prolonged oxidative stress and implications for its physiological role in Escherichia coli
6. Reactions of the Escherichia coli flavohaemoglobin (Hmp) with oxygen and reduced nicotinamide adenine dinucleotide: Evidence for oxygen switching of flavin oxidoreduction and a mechanism for oxygen sensing
7. Reactions of the Escherichia coil flavohaemoglobin (Hmp) with NADH and near-mioromolar oxygen: Oxygen affinity of NADH oxidase activity
8. The fiavohaemoglobin (HMP) of Escherichia coli generates superoxide in vitro and causes oxidative stress in vivo
9. Nitric oxide, nitrite, and fnr regulation of hmp (flavohemogiobin) gene expression in Escherichia coli K-12
10. A novel mechanism for upregulation of the Escherichia coli K-12 hmp (flavohaemoglobin) gene by the 'NO releaser', S-nitrosoglutathione: Nitrosation of homocysteine and modulation of MetR binding to the glyA-hmp intergenic region
11. The flavohemoglobin of Escherichia coli confers resistance to a nitrosating agent, a 'nitric oxide releaser,' and paraquat and is essential for transcriptional responses to oxidative stress
12. Primary sequence and evidence for a physiological function of the flavohermoprotein of Alcaligenes eutrophus
13. Flavohemoglobin Hmp affords inducible protection for Escherichia coli' respiration, catalyzed by cytochromes bd or bd, from nitric oxide
14. Role for the Salmonella flavohemoglobin in protection from nitric oxide
15. Nitric oxide dioxygenase: An enzymic function for flavohemoglobin
16. Nitrosative stress: Metabolic pathway involving the flavohemoglobin
17. Steady-state and transient kinetics of Eschrichia coli-nitric-oxide dioxygenase (flavohemoglobin). The B10 tyrosine hydroxyl is essential for dioxygen binding and catalysis
18. Anoxic function for the Escherichia coli flavohaemoglobin (Hmp): Reversible binding of nitric oxide and reduction to nitrous oxide
19. Paraquat regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12 is SoxRS independent but modulated by σ
20. Stable high-copy-number bacteriophage λ promoter vectors for overproduction of proteins in Escherichia coli
21. Ubiquinone limits oxidative stress in Escherichia coli
22. Spectroscopic studies on an oxygen-binding haemoglobin-like flavohaemoprotein from Escherichia coli
23. 1 b' preparation
24. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
25. Escherichia coli flavohaemoglobin (Hmp) reduces cytochrome c and Fe(III) hydroxamate Κ by electron transfer from NADH via FAD: Sensitivity of oxidoreductase activity to haem-bound dioxygen
26. Superoxide modulates the oxidation and nitrosation of thiols by nitric oxide-derived reactive intermediates
27. Constitutive and adaptive detoxification of nitric oxide in Escherichia coli. Role of nitric-oxide dioxygenase in the protection of aconitase
28. Oxygen-dependent regulation of the respiration and growth of Escherichia coli by nitric oxide
29. Microbial ecology of the gastrointestinal tract
30. The respiratory chains of Escherichia coli
31. Redundancy of aerobic respiratory chains in bacteria? Routes, reasons and regulation