Reduced activity of BamHI variants C54I, C64W, and C54D/C64R is consistent with the substrate-assisted catalysis model

Biochemical and Biophysical Research Communications

Volumn 287, Issue 1, 2001, Pages 153-159

  Acharya, A S ^a   Roy, K B ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

BamHI variants; Cys54 mutation; Site.specific mutagenesis

Indexed keywords

MUTANT PROTEIN; RESTRICTION ENDONUCLEASE;

ARTICLE; CATALYSIS; ENZYME KINETICS; ENZYME SUBSTRATE; GENE MUTATION; MODEL; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

EID: 0035860243     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5558     Document Type: Article

References (18)

1. Recognition sequence of specific endonuclease BamHI from Bacillus amyloliquefaciens
2. Effect of sulfhydryl group inhibitors on restriction endonuclease activities
3. Structure of BamHI endonuclease bound to DNA: Partial folding and unfolding on DNA binding
4. Cofactor requirement of BamHI mutant endonuclease E77K and its suppressor mutants
5. Substrate assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes
6. Evidence for substrate assisted catalysis in the DNA cleavage of several endonucleases
7. The role of metals in catalysis by the restriction endonuclease BamHI
8. Recognition and cleavage of DNA by type II restriction endonucleases
9. Protein engineering of BamHI restriction endonuclease: Replacement of Cys54 by Ala enhances catalytic activity
10. The megaprimer method of site directed mutagenesis
11. An alternative approach for screening active BamHI variants: Overexpression in T7 RNA polymerase based system
12. SOS induction as an in-vivo assay of enzyme-DNA interaction
13. A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding
14. Continuous spectrophotometric assay for restriction endonuclease using oligonucleotide and based on hyperchromic effect
15. DNA damaging agents stimulate gene expression at specific loci in Escherichia coli
16. Weakly polar interactions in proteins
17. Type II restriction endonucleases: Structural, functional and evolutionary relationships