Two new thermostable type II restriction endonucleases from Thermus aquaticus: TatI and TauI, which recognize the novel nucleotide sequences 5′-W↓GTACW-3′ and 5′-GCSG↓C-3′ respectively

FEMS Microbiology Letters

Volumn 204, Issue 2, 2001, Pages 253-257

  Vitkute, J ^a   Maneliene, Z ^a   Janulaitis, A ^a  

^a VILNIUS UNIVERSITY   (Lithuania)

Author keywords

Restriction endonuclease; Thermostable; Thermus

Indexed keywords

DNA FRAGMENT; RESTRICTION ENZYME ACEIII; RESTRICTION ENZYME BBVI; RESTRICTION ENZYME BGLI; RESTRICTION ENZYME BSEPI; RESTRICTION ENZYME FNUDII; RESTRICTION ENZYME HGIAI; RESTRICTION ENZYME MAEII; RESTRICTION ENZYME MBOI; RESTRICTION ENZYME MSEI; RESTRICTION ENZYME PVUII; RESTRICTION ENZYME STUI; RESTRICTION ENZYME TAQI; RESTRICTION ENZYME TATI; RESTRICTION ENZYME TAUI; RESTRICTION ENZYME TSP4CI; RESTRICTION ENZYME TSPEI; RESTRICTION ENZYME XHOI; RESTRICTION ENZYME XMAIII; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM ISOLATE; CONTROLLED STUDY; DNA CLEAVAGE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ISOSCHIZOMER; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; THERMOSTABILITY; THERMUS AQUATICUS;

BASE SEQUENCE; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA; ENZYME STABILITY; HEAT; SUBSTRATE SPECIFICITY; THERMUS;

THERMUS; THERMUS AQUATICUS;

EID: 0035856597     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(01)00410-4     Document Type: Article

References (13)

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3. New restriction endonucleases from thermophilic soil bacteria
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6. Two thermostable type II restriction endonucleases from Icelandic strains of the genus Thermus: Tsp4CI (ACN/GT), a novel type II restriction endonuclease, and Tsp8EI, an isoschizomer of the mesophilic enzyme BglI (GCCNNNN/NGGC)
7. A new restriction endonuclease, TspEI, from the genus Thermus that generates cohesive termini compatible with those of EcoRI
8. Tsp45I, a new thermostable site-specific endonuclease that cleaves the recognition sequence 5′-↓GTSAC-3′
9. A site-specific endonuclease from Thermus thermophilus 111, Tth111I
10. A second site specific endonuclease from Thermus thermophilus 111, Tth111II
11. Bacteria of gender Thermus and their thermostable enzymes
12. Isoschizomers of the restriction endonuclease TaqI (T/CGA) requiring different metal ion concentrations and having a range of thermal stabilities from Thermus species from different continents
13. Genetic disease detection and DNA amplification using cloned thermostable ligase