Fluorescently labeled model DNA sequences for exonucleolytic sequencing

Journal of Biotechnology

Volumn 86, Issue 3, 2001, Pages 203-224

  Földes Papp, Zeno ^a,d   Angerer, Bernhard ^b   Thyberg, Per ^a   Hinz, Michael ^c   Wennmalm, Stefan ^a   Ankenbauer, Waltraud ^b   Seliger, Hartmut ^c   Holmgren, Arne ^a   Rigler, Rudolf ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b ROCHE DIAGNOSTICS GMBH   (Germany)

^c UNIVERSITY OF ULM   (Germany)

^d UNIVERSITY OF GRAZ   (Austria)

Author keywords

Base specific incorporation; Covalent incorporation; Exonucleolytic DNA sequencing; Fluorophore bearing nucleotides; Mobility shift gel electrophoresis; Organic extraction; PCR based labeling (tagging); Re sequencing; Reversion PCR; Solid phase labeling (tagging); Solution phase labeling (tagging); Synthetic, tailor made DNAs; T7 DNA polymerase; Thioredoxin; Two color fluorescence cross correlation spectroscopy

Indexed keywords

DNA POLYMERASE; EXONUCLEASE; THIOREDOXIN;

ARTICLE; COVALENT BOND; DNA CONFORMATION; DNA PURIFICATION; DNA SEQUENCE; DNA TEMPLATE; ENZYME ACTIVITY; FLUORESCENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SPECTROSCOPY;

BASE SEQUENCE; DNA; FLUORESCENT DYES; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RHODAMINES; SEQUENCE ANALYSIS, DNA; SPECTROMETRY, FLUORESCENCE; TAQ POLYMERASE; TEMPLATES, GENETIC;

EID: 0035853636     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(00)00414-4     Document Type: Article

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