The use of a second reporter plasmid as an internal standard to normalize luciferase activity in transient transfection experiments may lead to a systematic error

Journal of Biotechnology

Volumn 88, Issue 3, 2001, Pages 251-258

  Huszár, Tamás ^a   Mucsi, István ^a   Terebessy, Tamás ^a   Masszi, András ^a   Adamkó, Sarolta ^a   Jeney, Csaba ^a   Rosivall, László ^a  

^a SEMMELWEIS UNIVERSITY   (Hungary)

Author keywords

Galactosidase vectors; Internal standard; Transient transfection

Indexed keywords

BODY FLUIDS; CELLS; ENZYMES; VIRUSES;

PLASMIDS;

BIOTECHNOLOGY;

ANGIOTENSIN; BACTERIAL ENZYME; BETA GALACTOSIDASE; LUCIFERASE; PROTEIN C FOS; THYMIDINE KINASE;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; CYTOMEGALOVIRUS; ENZYME ACTIVITY; ERROR; GENE CONSTRUCT; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPORTER GENE; ROUS SARCOMA ONCOVIRUS; STANDARD;

ANGIOTENSIN II; ANIMALS; ANTINEOPLASTIC COMBINED CHEMOTHERAPY PROTOCOLS; BETA-GALACTOSIDASE; CALCIUM PHOSPHATES; CELL LINE; CRICETINAE; CRICETULUS; CYCLOPHOSPHAMIDE; DOXORUBICIN; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENES, FOS; GENES, REPORTER; LUCIFERASES; PLASMIDS; RATS; RECEPTOR, ANGIOTENSIN, TYPE 1; RECEPTORS, ANGIOTENSIN; REFERENCE STANDARDS; SIGNAL TRANSDUCTION; TRANSFECTION; VINCRISTINE;

EID: 0035850254     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(01)00277-2     Document Type: Article

References (13)

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