Ribosomes with large synthetic N-terminal extensions of protein S15 are active in vivo

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1544, Issue 1-2, 2001, Pages 378-385

  Walles Granberg, A ^a   Schnell, R ^a,b   Isaksson, L A ^a   Ryden Aulin M ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b BAY ZOLTÁN NONPROFIT LTD FOR APPLIED RESEARCH   (Hungary)

Author keywords

Protein fusion; Ribosomal protein S13; Ribosomal protein S15; Ribosomal protein S4; Ribosome structure

Indexed keywords

HYBRID PROTEIN; MUTANT PROTEIN; PROTEIN S 4; RIBOSOMAL PROTEIN S13; RIBOSOMAL PROTEIN S15; RIBOSOME PROTEIN; STAPHYLOCOCCUS PROTEIN A; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL MUTATION; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; GENE FUSION; GENETIC TRANSFORMATION; IN VIVO STUDY; NONHUMAN; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; REPORTER GENE; RIBOSOME SUBUNIT; STAPHYLOCOCCUS AUREUS;

BASE SEQUENCE; CHROMATOGRAPHY, LIQUID; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; RIBOSOMAL PROTEINS; RIBOSOMES; STAPHYLOCOCCAL PROTEIN A;

ESCHERICHIA COLI;

EID: 0035846963     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(00)00252-1     Document Type: Article

References (28)

1. The three-dimensional structure of the ribosome and its components
2. Placement of protein and RNA structures into a 5 Å-resolution map of the 50S ribosomal subunit
3. X-Ray crystal structures of 70S ribosome functional complexes
4. Identification of an RNA-protein bridge spanning the ribosomal subunit interface
5. Conformational variability in Escherichia coli 70S ribosome as revealed by 3D cryo-electron tomography
6. Three-dimensional structure of a specific pre-messenger RNP particle established by electron microscope tomography
7. Electron microscope tomography
8. Starvation in vivo for aminoacyl-tRNA increases the spatial separation between the two ribosomal subunits
9. Physiologically dependent appearance of a low density region that corresponds to the tunnel through the 50S part of the 70S ribosome
10. Visualization of a large conformation change of ribosomes in Escherichia coli cells starved for tryptophan or treated with kirromycin
11. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage Lambda and Mu
12. Derivatives of the yeast mitochondrial ribosomal protein MrpS28 replace ribosomal protein S15 as functional components of the Escherichia coli ribosome
13. Genetic studies of the lac repressor. III. Additional correlation of mutational sites with specific amino acid residues
14. Culture medium for enterobacteria
15. Approaches to solid phase DNA sequencing
16. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support
17. A synthetic IgG-binding domain based on staphylococcal protein A
18. Structure of the C-terminal end of the nascent peptide influences translation termination
19. Ribosome activity and modification of 16S RNA are influenced by deletion of ribosomal protein S20
20. 6A37 in transfer RNA, mutation in rpsD(S4) or streptomycin
21. Suppression of the Escherichia coli rpoH opal mutation by ribosomes lacking S15 protein
22. Structure of a bacterial 30S ribosomal subunit at 5.5 Å resolution
23. Solution structure of the ribosomal RNA binding protein from Thermus thermophilus
24. Conformational variability of the N-terminal helix in the structure of ribosomal protein S15
25. Structure of the S15,S6,S18-rRNA complex: Assembly of the 30S ribosome central domain
26. The mechanism of binding staphylococcal protein A to immunoglobulin G does not involve helix unwinding