Purification and crystallization of CII: An unstable transcription activator from phage λ

Biochemical and Biophysical Research Communications

Volumn 288, Issue 4, 2001, Pages 997-1000

  Datta, A B ^a   Chakrabarti, P ^a   Subramanya, H S ^b   Parrack, P ^a  

^a BOSE INSTITUTE   (India)

^b CENTRAL DRUG RESEARCH INSTITUTE   (India)

Author keywords

Bacteriophage lambda; CII protein; Crystallization; Genetic switch; Lysogeny

Indexed keywords

AMMONIUM SULFATE;

ARTICLE; BACTERIOPHAGE LAMBDA; CRYSTAL STRUCTURE; CRYSTALLIZATION; DIFFUSION; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; ION EXCHANGE CHROMATOGRAPHY; LYSOGENIZATION; MOLECULAR CLONING; NONHUMAN; PH; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PURIFICATION; VAPOR;

BACTERIOPHAGE LAMBDA; ESCHERICHIA COLI;

EID: 0035834559     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5880     Document Type: Article

References (23)

1. Bacteriophage λ development: Temporal switches and the choice of lysis or lysogeny
2. The lysis-lysogeny decision of phage λ: Explicit programming and responsiveness
3. Purification and properties of a transcriptional activator: The cII protein of phage λ
4. Proteolysis of the phage lambda CII regulatory protein by FtsH (HflB) of Escherichia coli
5. Control of phage lambda development by stability and synthesis of cII protein: Role of the viral cIII and host hflA, himA and himD genes
6. Proteolysis of bacteriophage lambda CII by Escherichia coli FtsH (HflB)
7. hflB, a new Escherichia coli locus regulating lysogeny and the level of bacteriophage lambda cII protein
8. Bacteriophage lambda protein cII binds promoters on the opposite face of the DNA helix from RNA polymerase
9. Regulation of replication of lambda phage and lambda plasmid DNAs at low temperature
10. Impaired lysogenisation of the Escherichia coli rpoA341 mutant by bacteriophage lambda is due to the inability of CII to act as a transcriptional activator
11. Drastically decreased transcription from CII-activated promoters is responsible for impaired lysogenization of the Escherichia coli rpoA341 mutant by bacteriophage lambda
12. RE promoter: Enhanced formation and stabilization of open complexes
13. Sparse matrix sampling: A screening method for crystallization of proteins
14. Recent changes to the MOSFLM package for processing film and image plate data
15. Collaborative Computational
16. Solvent content of protein crystals
17. Identification of the DNA binding domain of the phage lambda cII transcriptional activator and the direct correlation of cII protein stability with its oligomeric forms
18. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
19. Selenomethionyl proteins produced for analysis by multiwave-length anomalous diffraction (MAD): A vehicle for direct determination of three-dimensional structure
20. Crystal structure of globular domain of histone H5 and its implications for nucleosome binding