Different susceptibility of the two dimers of ribonuclease A to subtilisin. Implications for their structure

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1545, Issue 1-2, 2001, Pages 255-262

  Nenci, Arianna ^a   Gotte, Giovanni ^a   Maras, Bruno ^b   Libonati, Massimo ^a  

^a UNIVERSITY OF VERONA   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Different subtilisin action on the two RNase A dimers; Dimeric RNase A; Dimeric RNase A conformer; S protein; Subtilisin

Indexed keywords

DIMER; PROTEIN SUBUNIT; RIBONUCLEASE A; SERINE; SUBTILISIN; VITRONECTIN;

AMINO TERMINAL SEQUENCE; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVITY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN STRUCTURE; THERMOSTABILITY;

ANIMALS; CATTLE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DIMERIZATION; PROTEIN CONFORMATION; RIBONUCLEASE, PANCREATIC; SERINE; SUBTILISIN;

EID: 0035830749     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(00)00287-9     Document Type: Article

References (14)

1. Preparation of ribonuclease S and its component parts, S-peptide and S-protein
2. Observation of a noncovalent ribonuclease S/S-peptide complex by electrospray ionization mass spectrometry
3. A study of the thermal denaturation of ribonuclease S by electrospray ionization mass spectrometry
4. Interaction of semisynthetic variants of RNase A with ribonuclease inhibitor
5. Evaluation of subtilisin cleavage specificity for RNase A peptide bonds by high-performance liquid chromatography and mass spectrometry analysis
6. On the aggregation of bovine pancreatic ribonuclease
7. Preparation and properties of two active forms of ribonuclease dimer
8. Two different forms of aggregated dimers of ribonuclease A
9. Structural versatility of bovine ribonuclease A. Distinct conformers of trimeric and tetrameric aggregates of the enzyme
10. The two dimeric forms of RNase A
11. The crystal structure of a 3D domain-swapped dimer of RNase A at 2.1 Å resolution
12. Resistance of ribonuclease A dimers to subtilisin
13. Polyspermine-ribonuclease prepared by cross-linkage with dimethyl suberimidate
14. A spectrophotometric method for measurement of ribonuclease activity