Protease C2, a cysteine endopeptidase involved in the continuing mobilization of soybean β-conglycinin seed proteins

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1545, Issue 1-2, 2001, Pages 192-206

  Seo, Sang Beom ^a,b   Tan Wilson, Anna ^a   Wilson, Karl A ^a  

^a BINGHAMTON UNIVERSITY   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Beta conglycinin; Cysteine protease; Glycine mex; Glycinin; Proteolysis; Soybean

Indexed keywords

BETA CONGLYCININ; PROTEINASE; PROTEINASE C2; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; IN VIVO STUDY; NUCLEOTIDE SEQUENCE; PH; PHYTOCHEMISTRY; PLANT SEED; PRIORITY JOURNAL; PROTEIN DEGRADATION; SEQUENCE ANALYSIS; SOYBEAN;

AMINO ACID SEQUENCE; CYSTEINE ENDOPEPTIDASES; GLOBULINS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PLANT PROTEINS; PROTEASE INHIBITORS; PROTEIN CONFORMATION; SEEDS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SOYBEAN PROTEINS; SOYBEANS; SUBSTRATE SPECIFICITY;

GLYCINE MAX; PHASEOLUS VULGARIS;

EID: 0035830722     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(00)00277-6     Document Type: Article

References (49)

1. Regulation of plant gene expression at the posttranslational level: Applications to genetic engineering
2. The structure and biosynthesis of legume seed storage proteins: A biological solution to the storage of nitrogen in seeds
3. Proteases and proteolytic cleavage of storage proteins in developing and germinating dicotyledonous seeds
4. Plant cysteine proteinases in germination and senescence
5. Degradation of storage proteins in germinating seeds
6. Purification of an endopeptidase involved with storage protein degradation in Phaseolus vulgaris L. cotyledons
7. A vacuolar cysteine endopeptidase (SH-EP) that digests seed storage globulin: Characterization, regulation of gene expression, and posttranslational processing
8. The two cysteine endopeptidases of legume seeds: Purification and characterization by use of specific fluorometric assays
9. Asparaginyl endopeptidase
10. Proteinase A, a storage globulin-degrading endopeptidase of vetch (Vicia sativa L.) seeds, is not involved in early steps of storage-protein mobilization
11. Molecular cloning and characterization of Vigna mungo processing enzyme 1 (VmPE-1), an asparaginyl endopeptidase possibly involved in post-translational processing of a vacuolar cysteine endopeptidase (SH-EP)
12. Does an asparaginyl-specific cysteine endopeptidase trigger phaseolin degradation in cotyledons of kidney bean seedlings?
13. Major proteins of soybean seeds. Subunit structure of β-conglycinin
14. Complete sequence of a cDNA of α subunit of soybean β-conglycinin
15. Differential proteolysis of glycinin and β-conglycinin polypeptides during soybean germination and seedling growth
16. Characterization of the major protease involved in the soybean β-conglycinin storage protein mobilization
17. An acidic amino acid-specific protease from germinating soybeans
18. Characterization of a soybean β-conglycinin-degrading protease cleavage site
19. Characterization of the glycinin gene family in soybean
20. The β-conglycinins of soybean remain assembled in a 7S conformation while undergoing proteolytic cleavage during germination and early seedling growth
21. Characterization of 30-kDa fragments derived from β-conglycinin degradation process during germination and seedling growth of soybean
22. Purification and characterization of protease C2, the enzyme that degrades the β subunit of β-conglycinin in soybean seedlings
23. Legumin and vicilin, storage proteins of legume seeds
24. Equal expression of the maternal and paternal alleles for the polypeptide subunits of the major storage protein of the bean Phaseolus vulgaris L.
25. A buffer solution for colorimetric comparison
26. Cleavage of structural proteins during assembly of the head of bacteriophage T4
27. Transcript levels for a mung bean cysteine protease during early seedling growth
28. Differential expression of soybean cysteine proteinase inhibitor genes during development and in response to wounding and methyl jasmonate
29. Copper staining: A five minute protein stain for sodium dodecyl sulfate polyacrylamide gels
30. Silver staining of proteins in polyacrylamide gels
31. Temporal and spacial expression of a thiolprotease gene during pea ovary senescence, and its regulation by gibberellin
32. Nucleotide sequences from phaseolin cDNA clones: The major storage proteins from Phaseolus vulgaris are encoded by two unique gene families
33. Contributing of processing events to the molecular heterogeneity of four banding types of phaseolin, the major storage protein of Phaseolus vulgaris L.
34. Three-dimensional structure of the seed storage protein phaseolin at 3 Å resolution
35. Structure of phaseolin at 2.2 Å resolution. Implications for a common vicilin/legumin structure and the genetic engineering of seed storage proteins
36. The glycosylated seed storage proteins of Glycine max and Phaseolus vulgaris. Structural homologies of genes and proteins
37. The amino-acid sequence in the phenylalanyl chain of insulin. 2. Investigation of peptides from enzymic hydrolysates
38. Survey of proteolytic activities degrading the Kunitz trypsin inhibitor and glycinin in germinating soybeans (Glycine max)
39. The roles of the N-linked glycans and extension regions of soybean beta-conglycinin in folding, assembly and structural features
40. Proteolysis of phaseolin in relation to its structure
41. In vitro enzymatic hydrolysis of phaseolin, the major storage protein of Phaseolus vulgaris L.
42. Initiation of the degradation of the soybean Kunitz and Bowman-Birk trypsin inhibitors by a cysteine protease
43. A protease responsible for post-translational cleavage of a conserved Asn-Gly linkage in glycinin, the major seed storage protein of soybean
44. Vacuolar processing enzyme of soybean that converts proproteins to the corresponding mature forms
45. In vivo endoproteolytically cleaved phaseolin is stable and accumulates in developing Phaseolus lunatus L. seeds
46. Degradation of the major storage protein of Phaseolus vulgaris during germination
47. Proteinase A-like enzyme from germinated kidney bean seeds. Its action on phaseolin and vicilin