Replication origins in Xenopus egg extract are 5-15 kilobases apart and are activated in clusters that fire at different times

Journal of Cell Biology

Volumn 152, Issue 1, 2001, Pages 15-25

  Blow, J Julian ^a   Gillespie, Peter J ^a   Francis, Dennis ^b   Jackson, Dean A ^c,d  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b CARDIFF UNIVERSITY   (United Kingdom)

^c UNIVERSITY OF MANCHESTER   (United Kingdom)

^d UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

ORC; Origin clusters; Replication origin; S phase; Xenopus

Indexed keywords

ARTICLE; CELL CYCLE S PHASE; DNA REPLICATION ORIGIN; DNA SEQUENCE; DNA STRUCTURE; EGG; EMBRYO DEVELOPMENT; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS; XENOPUS;

ANIMALS; AUTORADIOGRAPHY; BASE PAIRING; BROMODEOXYURIDINE; CELL EXTRACTS; CELL NUCLEUS; DNA REPLICATION; FEMALE; FERTILIZATION; MALE; OVUM; REPLICATION ORIGIN; SPERMATOZOA; TIME FACTORS; XENOPUS;

EID: 0035825156     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.152.1.15     Document Type: Article

References (39)

1. Preventing re-replication of DNA in a single cell cycle: Evidence for a replication licensing factor
2. Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs
3. A role for the nuclear envelope in controlling DNA replication within the cell cycle
4. Replication of purified DNA in Xenopus egg extract is dependent on nuclear assembly
5. Nuclei act as independent and integrated units of replication in a Xenopus cell-free system
6. The units of DNA replication in Drosophila melanogaster chromosomes
7. A relationship between replicon size and supercoiled loop domains in the eukaryotic genome
8. Replication of DNA in the chromosomes of eukaryotes
9. The role of MCM/P1 proteins in the licensing of DNA replication
10. Purification of an Mcm-containing complex as a component of the DNA replication licensing system
11. Characterization of the Xenopus replication licensing system
12. The nucleoskeleton and the topology of replication
13. Once and only once upon a time: Specifying and regulating origins of DNA replication in eukaryotic cells
14. Nucleoplasmin-mediated chromatin remodelling is required for Xenopus sperm nuclei to become licensed for DNA replication
15. The regulation of DNA synthesis and mitosis in multinucleate frog eggs
16. Regulation of DNA replication on subchromosomal units of mammalian cells
17. Eucaryotic DNA: Organization of the genome for replication
18. A cytoplasmic clock with the same period as the division cycle in Xenopus eggs
19. Regulated DNA replication of DNA microinjected into eggs of Xenopus laevis
20. Replication fork density increases during DNA synthesis in X. laevis egg extracts
21. Plasmid replication in Xenopus eggs and egg extracts: A 2D gel electrophoretic analysis
22. Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos
23. Transition in specification of embryonic metazoan DNA replication origins
24. Role of nuclear architecture in the initiation of eukaryotic DNA replication
25. Replicon clusters are stable units of chromosome structure: Evidence that nuclear organization contributes to the efficient activation and propagation of S phase in human cells
26. Replicon size, mean rate of DNA replication and the duration of the cell cycle and its component phases in eight monocotyledons of contrasting DNA C values
27. The events of the midblastula transition in Xenopus are regulated by changes in the cell cycle
28. Chromosome replication in early development of Xenopus laevis
29. Mechanisms ensuring rapid and complete DNA replication despite random initiation in Xenopus early embryos
30. DNA replication initiates at multiple sites on plasmid DNA in Xenopus egg extracts
31. Lack of specific sequence requirement for DNA replication in Xenopus eggs compared with high sequence specificity in yeast
32. Nuclear reconstitution in vitro: Stages of assembly around protein-free DNA
33. High resolution visual mapping of stretched DNA by fluorescent hybridization
34. Interaction between the origin recognition complex and the replication licensing system in Xenopus
35. Changes in association of the Xenopus origin recognition complex with chromatin on licensing of replication origins
36. DNA fiber replication in chromosomes of a higher plant (Pisum sativum)
37. The size and number of replicon families of chromosomal DNA of Arabidopsis thaliana
38. Regulation of replicon size in Xenopus egg extracts