On-line monitoring of a quasi-enantiomeric reaction with two coumarin substrates via 2D-fluorescence spectroscopy

Enzyme and Microbial Technology

Volumn 29, Issue 2-3, 2001, Pages 150-159

  Knüttel, Torsten ^a   Hartmann, Thorsten ^a   Meyer, Hartmut ^a   Scheper, Thomas ^a  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

Author keywords

2D fluorescence; Chymotrypsin; Coumarin; Esterase; On line monitoring

Indexed keywords

FLUORESCENCE; HYDROLYSIS; SPECTROSCOPIC ANALYSIS; SUBSTRATES;

QUASI-ENANTIOMERIC REACTIONS;

ENZYME KINETICS;

CHYMOTRYPSIN A; COUMARIN DERIVATIVE; DEXTRO PHENYLALANIN 7 AMIDO 4 TRIFLUOROMETHYLCOUMARIN; ESTERASE; PHENYLALANIN 7 AMIDO 4 METHYLCOUMARIN; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; ASSAY; CATTLE; CHEMICAL STRUCTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENANTIOMER; ENZYME MECHANISM; FLUORESCENCE SPECTROSCOPY; HYDROLYSIS; KINETICS; LIVER; MONITORING; NONHUMAN; SWINE;

SUIDAE;

EID: 0035822718     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(01)00369-6     Document Type: Article

References (13)

1. Lipase-catalyzed esterification of 2-(4-substituted phenoxy)propionic acids in organic solvents: Substituent controlling enantioselectivity toward racemic acids
2. Direct photometric or fluorometric assay of proteinases using substrates containing 7-amino-4-trifluoromethylcoumarin
3. Bioreactor characterisation by means of in situ fluorimetry
4. Two new fluorogenic substrates for the detection of Penicilin-G-Acylase activity
5. A new fluorogenic substrate for chymotrypsin
6. Sensitive assasy for trypsin, elastase and chymotrypsin using new fluorogenic substrates
7. Factors affecting culture fluorescence when monitoring bioreactors