Biochemical and biophysical demonstration of GPCR oligomerization in mammalian cells

Life Sciences

Volumn 68, Issue 19-20, 2001, Pages 2243-2250

  Angers, S ^a   Salahpour, A ^a   Bouvier, M ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Energy transfer; G protein coupled receptor; Oligomerization

Indexed keywords

BETA 2 ADRENERGIC RECEPTOR; EPITOPE; G PROTEIN COUPLED RECEPTOR;

ARTICLE; BIOLUMINESCENCE; CELL LINE; CONTROLLED STUDY; DRUG SCREENING; EMBRYO CELL; ENERGY TRANSFER; FIBROBLAST; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; KIDNEY CELL; OLIGOMERIZATION; PROTEIN STRUCTURE; REGULATORY MECHANISM; STRUCTURE ANALYSIS;

EID: 0035815174     PISSN: 00243205     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0024-3205(01)01012-8     Document Type: Conference Paper

References (17)

1. Bargmann CI. - Neurobiology of the caenorhabditis elegans genome. - Science 1998 Dec 11;282(5396):2028-33 2028-2333.
2. Kobilka BK. Adrenergic receptors as models for G protein-coupled receptors. Annu.Rev.Neurosci. 1992; 15:87-114.
3. Salahpour A, Angers S, Bouvier M. - Functional significance of oligomerization of G-protein-coupled receptors. - Trends Endocrinol Metab 2000 Jul;11(5):163-168.
4. Hebert TE, Moffett S, Morello JP, et al. A peptide derived from a β2-adrenergic receptor transmembrane domain inhibits both receptor dimerization and activation. J.Biol.Chem. 1996;271:16384-16392.
5. Xu Y, Piston DW, Johnson CH. A bioluminescence resonance energy transfer (BRET) system: Application to interacting circadian clock proteins. Proceedings of the National Academy of Sciences of the United States of America 1999;96:151-156.
6. Zhu X, Wess J. Truncated V2 vasopressin receptors as negative regulators of wild-type V2 receptor function. Biochemistry 1998;37:15773-15784.
7. Benkirane M, Jin DY, Chun RF, Koup RA, Jeang KT. Mechanism of transdominant inhibition of CCR5-mediated HIV-1 infection by ccr5delta32. J.Biol.Chem. 1997;272:30603-30606.
8. Bai M, Quinn S, Trivedi S, Expression and characterization of inactivating and activating mutations in the human Ca2+o-sensing receptor. J.Biol.Chem. 1996;271:19537-19545.
9. Hebert TE, Loisel TP, Adam L, Ethier N, Onge SS, Bouvier M. Functional rescue of a constitutively desensitized beta2AR through receptor dimerization. Biochem.J. 1998;330:287-293.
10. Kaupmann K, Malitschek B, Schuler V, et al. GABA(B)-receptor subtypes assemble into functional heteromeric complexes. Nature 1998;396:683-687.
11. White JH, Wise A, Main M J, et al. Heterodimerization is required for the formation of a functional GABA(B) receptor. Nature 1998;396:679-682.
12. Jones KA, Borowsky B, Tamm JA, et al. GABA(B) receptors function as a heteromeric assembly of the subunits GABA(B)R1 and GABA(B)R2. Nature 1998;396:674-679.
13. Angers S, Salahpour A, joly E, et al. Detection of beta2-Adrenergic receptor dimerization in living cells using Bioluminescence Resonance Energy Transfer (BRET). Proc.Natl.Acad.Sci.USA 2000;In Press:
14. Rocheville M, Lange DC, Kumar U, Patel SC, Patel RC, Patel YC. - Receptors for dopamine and somatostatin: Formation of hetero-oligomers with enhanced functional activity. - Science 2000 Apr 7;288(5463): 154-7 154-177.
15. Overton MC, Blumer KJ. - G-protein-coupled receptors function as oligomers in vivo. - Curr Biol 2000 Mar 23;10(6):341-4 341-344.
16. Cornea A, Janovick JA, Maya-Nunez G, Conn PM. - Gonadotropin releasing hormone microaggregation: Rate monitored by fluorescence resonance energy transfer. - J Biol Chem 2000 Oct 16;: :
17. 1 -globin gene in a new SV40 host-vector system. CELL 1981;27:279-288.