Influence of water activity on the enantioselective esterification of (R,S)-ibuprofen by crosslinked crystals of Candida antarctica lipase B in organic solvent media

Biotechnology and Bioengineering

Volumn 75, Issue 5, 2001, Pages 559-562

  Pepin, Pierre ^a   Lortie, Robert ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Crosslinked crystals; Enantioselectivity; Enzyme; Lipase; Solvent; Water activity

Indexed keywords

CROSSLINKING; CRYSTALLIZATION; ESTERIFICATION; MICROORGANISMS;

MICROCRYSTALS;

ENZYME IMMOBILIZATION;

ACRYLIC ACID RESIN; GLUTARALDEHYDE; IBUPROFEN; LAURYL ALCOHOL; LIPASE B; ORGANIC COMPOUND; SOLVENT; WATER;

ARTICLE; CANDIDA ANTARCTICA; CATALYSIS; CONTROLLED STUDY; CROSS LINKING; CRYSTAL; CRYSTALLIZATION; ENANTIOMER; ENZYME PURIFICATION; ESTERIFICATION; NONHUMAN; REACTION ANALYSIS;

CANDIDA; CROSS-LINKING REAGENTS; CRYSTALLIZATION; DODECANOL; ESTERIFICATION; IBUPROFEN; LIPASE; OCTANES; STEREOISOMERISM; SUBSTRATE SPECIFICITY; WATER;

EID: 0035814367     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10051     Document Type: Article

References (14)

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
2. Scale-up of a chiral resolution using cross-linked enzyme crystals
3. Lipase-catalyzed enantioselective esterification of ibuprofen in organic solvents under controlled water activity
4. Chiral high performance liquid chromatography resolution of ibuprofen esters
5. Cross-linked enzyme crystals as highly active catalysts in organic solvents
6. Cross-linked crystals of Candida rugosa lipase: Highly efficient catalysts for the resolution of chiral esters
7. Novel crystalline catalysts
8. On the interracial activation of Candida antarctica (lipases A and B as compared to Humicola lanuginosa lipase
9. Reaction rates in organic media show similar dependence on water activity with lipase catalyst immobilized on different supports
10. Effects of changes in some solvent parameters on carboxypeptidase a in solution and in cross-linked crystals
11. Intermolecular cross linking of a protein in the crystalline state: Carboxypeptidase A
12. The enzymatic behavior of carboxypeptidase-A in the solid state
13. Cross-linked enzyme crystals as robust biocatalysts
14. The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica