In vitro activity of MEKK2 and MEKK3 in detergents is a function of a valine to serine difference in the catalytic domain

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1547, Issue 1, 2001, Pages 167-173

  Widmann, Christian ^a,b   Sather, Susan ^a,b   Oyer, Ryan ^a,b   Johnson, Gary L ^a,b   Dreskin, Stephen C ^a,b  

^a NATIONAL JEWISH MEDICAL AND RESEARCH CENTER   (United States)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

Detergent; Kinase; Kinase domain; MEKK; Mitogen activated protein kinase kinase

Indexed keywords

DETERGENT; LYSINE; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE KINASE; PROTEIN KINASE; SERINE; STRESS ACTIVATED PROTEIN KINASE; THREONINE; TRITON X 100; TRITON X 114; VALINE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; AUTOPHOSPHORYLATION; CATALYSIS; CELL LINE; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVE SITE; ENZYME ASSAY; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; MUTATION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BINDING SITES; CATALYSIS; CELL LINE; DETERGENTS; ENZYME STABILITY; HUMANS; LYSINE; MAP KINASE KINASE KINASE 2; MAP KINASE KINASE KINASE 3; MAP KINASE KINASE KINASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OCTOXYNOL; POLYETHYLENE GLYCOLS; SERINE; TRANSFECTION; VALINE;

EID: 0035810701     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(01)00183-2     Document Type: Article

References (9)

1. Blank J.L., Gerwins P., Elliott E.M., Sather S., Johnson G.L. Molecular cloning of mitogen-activated protein/ERK kinase kinases (MEKK) 2 and 3. Regulation of sequential phosphorylation pathways involving mitogen-activated protein kinase and c-Jun kinase. J. Biol. Chem. 271:1996;5361-5368.
2. Deacon K., Blank J.L. Characterization of the mitogen-activated protein kinase kinase 4 (MKK4)/c-Jun NH2-terminal kinase 1 and MKK3/p38 pathways regulated by MEK kinases 2 and 3. MEK kinase 3 activates MKK3 but does not cause activation of p38 kinase in vivo. J. Biol. Chem. 272:1997;14489-14496.
3. Widmann C., Dolci W., Thorens B. Internalization and homologous desensitization of the GLP-1 receptor depend on phosphorylation of the receptor carboxyl tail at the same three points. Mol. Endocrinol. 11:1997;1094-1102.
4. Burki E., Anjard C., Scholder J.C., Reymond C.D. Isolation of two genes encoding putative protein kinases regulated during Dictyostelium discoideum development. Gene. 102:1991;57-65.
5. Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.C., Coster F., Cziepluch C., De Haan M., Domdey H., Durand P., Entian K.D., Gatius M., Goffeau A., Grivell A., Hennemann L.A., Herbert C.J., Heumann K., Hilger F., Hollenberg C.P., Huang M.E., Jacq C., Jauniaux J.C., Katsoulou C., Karpfinger-Hartl L., et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome. EMBO J. 15:1996;2031-2049.
6. Posas F., Saito H. Osmotic activation of the HOG MAPK pathway via Ste11pMAPKKK: scaffold role of Pbs2p MAPKK. Science. 276:1997;1702-1705.
7. Helenius A., McCaslin D.R., Fries E., Tanford C. Properties of detergents. Methods Enzymol. 56:1979;734-749.
8. E. Harlow, D. Lane, Antibodies. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1988, p. 689.
9. S.M. Bhairi, Detergents. A Guide to the Properties and Uses of Detergents in Biological Systems, Calbiochem-Novabiochem Corp., La Jolla, CA, 1997.