Localization of the protein L2 in the 50 S subunit and the 70 S E. coli ribosome

Journal of Molecular Biology

Volumn 305, Issue 1, 2001, Pages 167-177

  Willumeit, Regine ^a   Forthmann, Stefan ^a   Beckmann, Jörn ^a,b   Diedrich, Gundo ^b   Ratering, Ralf ^c   Stuhrmann, Heinrich B ^a   Nierhaus, Knud H ^b  

^a INSTITUTE OF MATERIALS RESEARCH   (Germany)

^b MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^c FRAUNHOFER INSTITUTE FOR APPLIED INFORMATION TECHNOLOGY FIT   (Germany)

Author keywords

Neutron scattering; Protein biosynthesis; Ribosomal protein L2; Spin contrast variation

Indexed keywords

PROTEIN L2; RIBOSOME PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ELECTRON SPIN RESONANCE; ESCHERICHIA COLI; NEUTRON SCATTERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; RIBOSOME SUBUNIT;

ESCHERICHIA COLI;

EID: 0035808304     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4289     Document Type: Article

References (49)

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2. The Escherichia coli large ribosomal subunit at 7.5 Å resolution
3. Placement of protein and RNA structures into a 5 Å-resolution map of the 50 S ribosomal subunit
4. X-ray crystal structures of 70 S ribosome functional complexes
5. Structure of a bacterial 30 S ribosomal subunit at 5.5 Å resolution
6. The small ribosomal subunit from Thermus thermophilus at 4.5 Å resolution: Pattern fittings and the identification of a functional site
7. Direct visualization of A-, P-, and E-site transfer RNAs in the E. coli ribosome
8. Met and fitting of L1 protein
9. Arrangement of tRNAs in pre- and posttranslocational ribosomes revealed by electron cryomicroscopy
10. Peptidyl transfer on the ribosome
11. The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution
12. The structural basis of ribosome activity in peptide bond synthesis
13. Catalysis of peptide bond formarion by 50 S ribosomal subunits from Escherichia coli
14. Unusual resistance of peptidyltransferase to protein extraction procedures
15. Mapping important nucleotides in the peptidyl transferase centre of 23 S rRNA using a random mutagenesis approach
16. Histidine 229 in the protein L2 is apparently essential for 50 S peptidyl transferase activity
17. Mutational analysis of two highly conserved UGG sequences of 23 S rRNA from Escherichia coli
18. Minimal set of ribosomal components for the reconstitution of the peptidyltransferase activity
19. Ribosomal proteins L15 and L16 are mere late assembly proteins of the large ribosomal subunit
20. Peptidyl transferase activity catalyzed by protein-free 23S ribosomal RNA remains elusive
21. Reconstitution of the 50 S subunit with in vitro-transcribed 23S rRNA: A new tool for studying peptidyltransferase
22. Phylogenetic relationship of organisms by ribosomal protein comparison
23. Ribosomal protein L2 is involved in the association of the ribosomal subunits, tRNA binding to A and P sites and peptidyl transfer
24. A complete mapping of the proteins in the small ribosomal subunit of Escherichia coli
25. Inter-protein distances within the large subunit from Escherichia-coli ribosomes
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27. A map of protein-rRNA distribution in the 70 S Escherichia coli ribosome
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29. Total reconstitution of 70 S ribosomes from Escherichia coli
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31. A model of the translational apparatus based on a three-dimensional reconstruction of the Escherichia coli ribosome
32. The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome
33. Functional implications of ribosomal protein L2 in protein biosynthesis as shown by in vivo replacement studies
34. Differential localization of two epitopes of Escherichia coli ribosomal protein L2 on the large ribosomal subunit by immune electron microscopy using monoclonal antibodies
35. Topography of the ribosomal proteins from Escherichia coli within the intact subunits as determined by immunoelectron microscopy and protein-protein cross-linking
36. Neutron-scattering analysis of structural and functional aspects of the ribosome: The strategy of the glassy ribosome
37. Parameters for the preparation of Escherichia coli ribosomes and ribosomal subunits active in tRNA binding
38. Reconstitution of ribosomes
39. Preparation of functional ribosomal complexes and the effect of buffer conditions on tRNA positions observed by cryoelectron microscopy
40. Ribosomes and polysomes
41. An improved method for 2-d gel electrophoresis: Analysis of mutationally altered ribosomal proteins of E. coli
42. Association of a ribonuclease with the 50 S ribosomal subunit of Escherichia coli MRE600
43. The in situ structure of ribosomal proteins from polarized neutron scattering
44. The polarized target station at GKSS
45. Mechanism of nuclear dynamic polarisation by electron-nucleus dipol coupling in solids
46. Direct localization of the tRNAs within the elongating ribosome by means of neutron scattering (proton-spin contrast-variation)
47. Localisation of proteins and tRNA-molecules in the 70 S ribosome of the E. coli bacteria with polarized neutron scattering
48. A model for the spatial arrangement of the proteins in the large subunit of the Escherichia coli ribosome