A new approach for point mutation detection based on a ligase chain reaction

Journal of Biochemical and Biophysical Methods

Volumn 50, Issue 1, 2001, Pages 79-89

  Demchinskaya, Anna V ^a   Shilov, Ilya A ^a   Karyagina, Anna S ^a   Lunin, Vladimir G ^a   Sergienko, Olga V ^a   Voronina, Olga L ^a   Leiser, Matthias ^b   Plobner, Lutz ^b  

^a ALL RUSSIA RESEARCH INSTITUTE OF AGRICULTURAL BIOTECHNOLOGY   (Russian Federation)

^b Hemmelrather Weg 201   (Germany)

Author keywords

Cleavase; Colorimetric detection; Ligase chain reaction; Point mutation detection

Indexed keywords

BIOTIN; CLEAVASE; DOUBLE STRANDED DNA; ENZYME; OLIGONUCLEOTIDE; STREPTAVIDIN; UNCLASSIFIED DRUG;

ACCURACY; ADSORPTION; ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; COLORIMETRY; DNA TEMPLATE; ENZYME ASSAY; LIGASE CHAIN REACTION; POINT MUTATION; PRIORITY JOURNAL; PROMOTER REGION; SOLID;

BASE SEQUENCE; BIOTIN; DNA LIGASES; DNA MUTATIONAL ANALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDODEOXYRIBONUCLEASES; GENETIC TECHNIQUES; MOLECULAR SEQUENCE DATA; MUTATION; OLIGONUCLEOTIDES; POINT MUTATION; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EID: 0035807754     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(01)00178-6     Document Type: Article

References (19)

1. A method to detect ras point mutation in small subpopulations of cells
2. Rapid and sensitive nonradioactive detection of mutant K-ras genes via "enriched" PCR amplification
3. The use of synthetic oligonucleotides as hybridization probes: II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA
4. Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides
5. Multiplex detection of single-nucleotide variations using molecular beacons
6. Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia
7. Detection of single DNA base differences by competitive oligonucleotide priming
8. PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes
9. Primer extension technique for the detection of single nucleotide in genomic DNA
10. Dot blot detection of point mutations with adjacently hybridizing synthetic oligonucleotide probes
11. A ligase-mediated gene detection technique
12. Genetic disease detection and DNA amplification using cloned thermostable ligase
13. Sequencing end-labeled DNA with base-specific chemical cleavages
14. Improving the fidelity of Thermus thermophilus DNA ligase
15. Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases
16. Enzyme amplification - A general method applied to provide an immunoassisted assay for placental alkaline phospatase
17. The risk of recurrent deep venous thrombosis among heterozygous carriers of both factor V Leiden and the G20210A prothrombin mutation
18. A candidate genetic risk factor for vascular disease: A common mutation in methylenetetrahydrofolate reductase
19. Molecular lesion in patients with medium-chain acyl-CoA dehydrogenase deficiency