Expression of E. coli araBAD operon encoding enzymes for metabolizing L-arabinose in Saccharomyces cerevisiae

Enzyme and Microbial Technology

Volumn 28, Issue 1, 2001, Pages 16-24

  Sedlak, Miroslav ^a   Ho, Nancy W Y ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

Fermentation; L arabinose; L arabitol; PCR; Production of ethanol; S. cerevisiae

Indexed keywords

CATALYST ACTIVITY; ESCHERICHIA COLI; ETHANOL; FERMENTATION; GENES; METABOLISM; PH EFFECTS; RNA; THERMAL EFFECTS; YEAST;

ARABINOSE; ARABITOL; POLYMERASE CHAIN REACTION;

ENZYMES;

ALCOHOL; ARABINITOL; ARABINOSE; BACTERIAL ENZYME; GLUCOSE 6 PHOSPHATE ISOMERASE; GLYCOLYTIC ENZYME; LEVO ARABINOSE ISOMERASE; LEVO RIBULOKINASE; LEVO RIBULOSE 5 PHOSPHATE 4 EPIMERASE; MESSENGER RNA; PHOSPHOGLYCERATE KINASE; PYRUVATE KINASE; UNCLASSIFIED DRUG; XYLULOSE;

ARTICLE; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; OPERON; PH; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

EID: 0035793151     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(00)00282-9     Document Type: Article

References (39)

1. Composition of cellulotic raw materials for sugar production
2. An intralaboratory comparison of the performance of ethanol-producing micro-organisms in a xylose-rich acid hydrolysate
3. The utilization of sugar by yeast
4. Arabinose utilization by xylose-fermenting, yeasts, and fungi
5. Genetically engineered Saccharomyces yeast capable of effective cofermentation of glucose and xylose
6. Enhanced cofermentation of glucose and xylose by recombinant Saccharomyces yeast strains in batch and continuous operating modes
7. Stable recombinant yeasts for fermenting xylose to ethanol
8. Molecular aspect of ara regulation
9. The organization of the araBAD operon of Escherichia coli
10. A new pathway of pentose metabolism
11. Utilization of xylose by bacteria, yeasts, and fungi
12. DNA transfection of Escherichia coli by electroporation
13. Cloning and characterization of the xyl genes from Escherichia coli
14. High efficiency transformation of yeast by electroporation
15. Qualitative detection of penicillinase produced by yeast strains carrying chimeric yeast-coli plasmids
16. Uptake and catabolism of D-xylose in Salmonela thyphimurium LT2
17. Spectrophotometric method for the determination of free pentose and pentose in nucleotides
18. Crystalline L-ribulose 5-phosphate 4-epimerase from Escherichia coli
19. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
20. A rapid alkalin extraction procedure for screening recombinant plasmid DNA
21. Single-step purification of shuttle vectors from yeast for high frequency back-transformation into E. coli
22. The isolation, characterization, and sequence of the pyruvate kinase gene of Saccharomyces cerevisiae
23. The structure, and regulation of phosphoglucose isomerase in Saccharomyces cerevisiae
24. Efficient expression of Saccharomyces cerevisiae PGK gene depends on an upstream activation sequence but does not require TATA sequences
25. Primer-directed enzymatic amplification of DNA with a thermostable polymerase
26. The design of primers for PCR
27. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension
28. Relationship between sugar structure and competition for the sugar transport system in baker's yeast
29. Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase
30. Improved yeast promoters for overexpression of genes in E. coli
31. Purification and properties of an L-arabinose isomerase from Escherichia coli
32. Fluorescence dye as monitor of internal pH in Escherichia coli cells
33. Intracellular pH of yeast measured with fluorescent probes
34. Cloning and improving the expression of Pichia stipitis xylose reductase gene in Saccharomyces cerevisiae
35. Xylulokinase activity in various yeast including S. cerevisiae containing the cloned gene
36. Cloning of yeast xylulokinase gene by complementation of E. coli and yeast mutations
37. The fermentation of xylose - An analysis of the expression of Bacillus and Actinoplanes xylose isomerase genes in yeast
38. Expression of Escherichia coli xylose isomerase in Saccharomyces cerevisiae