Crystallization and initial crystallographic analysis of the disulfide bond isomerase DsbC in complex with the α domain of the electron transporter DsbD

Journal of Structural Biology

Volumn 136, Issue 2, 2001, Pages 162-166

  Haebel, Peter W ^a   Wichman, Steven ^a   Goldstone, David ^a   Metcalf, Peter ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

Crystal dehydration; Disulfide bond isomerase; DsbC; DsbD; Reaction intermediate; Thiol oxidoreductase; Transmembrane electron transporter; X ray crystallography

Indexed keywords

CARRIER PROTEIN; ISOMERASE; MUTANT PROTEIN; SOLVENT;

ARTICLE; CELL VOLUME; COMPLEX FORMATION; COOLING; CRYSTALLIZATION; CRYSTALLOGRAPHY; DESICCATION; DIFFRACTION; DISULFIDE BOND; ELECTRON TRANSPORT; ENZYME ACTIVE SITE; ENZYME STRUCTURE; MUTANT; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION;

ESCHERICHIA COLI;

EID: 0035782685     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsbi.2001.4430     Document Type: Article

References (18)

1. Bader, M. W., Xie, T., Yu, C. A., and Bardwell, J. C. (2000) Disulfide bonds are generated by quinone reduction. J. Biol. Chem. 275, 26082-26088.
2. Chung, J., Chen, T., and Missiakas, D. (2000) Transfer of electrons across the cytoplasmic membrane by DsbD, a membrane protein involved in thiol-disulphide exchange and protein folding in the bacterial periplasm. Mol. Microbiol. 35, 1099-1109.
3. Collaborative Computing Project No.4 (CCP4) (1994) The CCP4 suites: Programs for protein crystallography. Acta Crystallogr. D Biol. Crystallogr. 50, 760-763.
4. Fabianek, R. A., Hennecke, H., and Thony-Meyer, L. (2000) Periplasmic protein thiol:disulfide oxidoreductases of Escherichia coli. FEMS Microbiol. Rev. 24, 303-316.
5. Frand, A. R., Cuozzo, J. W., and Kaiser, C. A. (2000) Pathways for protein disulphide bond formation. Trends Cell Biol. 10, 203-210.
6. Goldstone, D., Haebel, P. W., Katzen, F., Bader, M. W., Bardwell, J. C., Beckwith, J., and Metcalf, P. (2001) DsbC activation by the N-terminal domain of DsbD. Proc. Natl. Acad. Sci. USA 98, 9551-9556.
7. Joly, J. C., and Swartz, J. R. (1997) In vitro and in vivo redox states of the Escherichia coli periplasmic oxidoreductases DsbA and DsbC. Biochemistry 36, 10067-10072.
8. Katzen, F., and Beckwith, J. (2000) Transmembrane electron transfer by the membrane protein DsbD occurs via a disulfide bond cascade. Cell 103, 769-779.
9. Martin, J. L. (1995) Thioredoxin - A fold for all reasons. Structure 3, 245-250.
10. McCarthy, A. A., Haebel, P. W., Torronen, A., Rybin, V., Baker, E. N., and Metcalf, P. (2000) Crystal structure of the protein disulfide bond isomerase, DsbC, from Escherichia coli. Nat. Struct. Biol. 7, 196-199.
11. Missiakas, D., Georgopoulos, C., and Raina, S. (1994) The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation. EMBO J. 13, 2013-2020.
12. Missiakas, D., Schwager, F., and Raina, S. (1995) Identification and characterization of a new disulfide isomerase-like protein (DsbD) in Escherichia coli. EMBO J. 14, 3415-3424.
13. Otwinowski, Z., and Minor, W. (1997) Processing of X-ray diffraction data collecting in oscillation mode. Methods Enzymol. 276, 307-326.
14. Powell, H. R. (1999) The Rossmann Fourier autoindexing algorithm in MOSFLM. Acta Crystallogr. D Biol. Crystallogr. 55, 1690-1695.
15. Rietsch, A., Bessette, P., Georgiou, G., and Beckwith, J. (1997) Reduction of the periplasmic disulfide bond isomerase, DsbC, occurs by passage of electrons from cytoplasmic thioredoxin. J. Bacteriol. 179, 6602-6608.
16. Shevchik, V. E., Condemine, G., and Robert-Baudouy, J. (1994) Characterization of DsbC, a periplasmic protein of Erwinia chrysanthemi and Escherichia coli with disulfide isomerase activity. EMBO J. 13, 2007-2012.
17. Stewart, E. J., Katzen, F., and Beckwith, J. (1999) Six conserved cysteines of the membrane protein DsbD are required for the transfer of electrons from the cytoplasm to the periplasm of Escherichia coli. EMBO J. 18, 5963-5971.
18. Zapun, A., Missiakas, D., Raina, S., and Creighton, T. E. (1995) Structural and functional characterization of DsbC, a protein involved in disulfide bond formation in Escherichia coli. Biochemistry 34, 5075-5089.