Improvement of the electro-transformation efficiency of facultatively alkaliphilic Bacillus pseudofirmus OF4 by high osmolarity and glycine treatment

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 12, 2001, Pages 2773-2775

  Ito, Masahiro ^a   Nagane, Makoto ^a  

^a TOYO UNIVERSITY   (Japan)

Author keywords

Alkaliphiles; Bacillus; Electroporation; Glycine treatment

Indexed keywords

BACILLUS PSEUDOFIRMUS;

GLYCINE;

ARTICLE; BACILLUS; CULTURE MEDIUM; ELECTROPORATION; METABOLISM; OSMOLARITY;

BACILLUS; CULTURE MEDIA; ELECTROPORATION; GLYCINE; OSMOLAR CONCENTRATION;

EID: 0035747748     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.2773     Document Type: Article

References (10)

1. Xue, G., Johnson, J. S., and Dalrumple, B. P., High osmolarity improves the electro-transformation efficiency of the Gram-positive bacteria Bacillus subtilis and Bacillus licheniformis. J. Microbial. Methods, 34, 183-191 (1999).
2. Buckley, N. D., Vadeboncoeur, C., Leblanc, D. J., Lee, N., and Frenette, M., An effective strategy, applicable to Streptococcus salivarius and related bacteria to enhance or confer Electroporation competence. Appl. Environ. Microbiol., 65, 3800-3804 (1999).
3. Dunny, G. M., Lee, L. N., and LeBlanc, D. J., Improved electroporation and cloning vector system for Gram-positive bacteria. Appl. Environ. Microbiol., 57, 1194-1201 (1991).
4. Holo, H. and Nes, I. F., High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media., Appl. Environ. Microbiol., 55, 3119-3123 (1989).
5. Stepanov, A. S., Puzanova, O. B., Dityatkin, O. G., Loginova, O. G., and Ilyashenko, B. N., Glycine-induced cryotransformation of plasmids in Bacillus anthracis., J. Gen. Microbiol., 136, 1217-1221 (1990).
6. Hammes, W., Schleifer, K. H., and Kandler, O., Mode of action of glycine on the biosynthesis of peptideglycan., J. Bacteriol., 116, 1029-1053 (1973).
7. + and alkali but not cholate resistance., J. Bacteriol., 182, 5663-5670 (2000).
8. + antiporter of alkaliphilic Bacillus firmus OF4., J. Bacteriol., 179, 3854-3857 (1997).
9. Gruss, A. and Ehrlich, S. D., The family of highly interrelated single-stranded deoxyribonucleic acid plasmids. Microbiol. Rev., 53, 231-241 (1989).
10. Aono, R. Isolation and partial characterization of structural components of the walls of alkaliphilic Bacillus strain C-125., J. Gen. Microbiol., 131, 105-111 (1985).