Novel substrate specificity of designer 3-isopropylmalate dehydrogenase derived from Thermus thermophilus HB8

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 12, 2001, Pages 2695-2700

  Fujita, Masaaki ^a   Tamegai, Hideyuki ^a   Eguchi, Tadashi ^a   Kakinuma, Katsumi ^a  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

3 isopropylmalate dehydrogenase; Designed catalytic reaction; Point mutation

Indexed keywords

THERMUS THERMOPHILUS;

3 ISOPROPYLMALATE DEHYDROGENASE; ALCOHOL DEHYDROGENASE; PRIMER DNA;

ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; NUCLEOTIDE SEQUENCE; SITE DIRECTED MUTAGENESIS; THERMUS THERMOPHILUS;

3-ISOPROPYLMALATE DEHYDROGENASE; ALCOHOL OXIDOREDUCTASES; BASE SEQUENCE; CATALYTIC DOMAIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA PRIMERS; MASS SPECTROMETRY; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY; THERMUS THERMOPHILUS;

EID: 0035747746     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.2695     Document Type: Article

References (23)

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