Quantification in mass units of group 1 grass allergens by a monoclonal antibody-based sandwich ELISA

Clinical and Experimental Allergy

Volumn 31, Issue 8, 2001, Pages 1271-1278

  Arilla, M C ^a   Ibarrola, I ^a   Eraso, E ^a   Aguirre, M ^a   Martinez A ^a   Asturias, J A ^a  

^a Research and Development Department   (Spain)

Author keywords

Lol p 1; Monoclonal antibody; Pollen allergy; Pollen extract; Recombinant allergen; Sandwich ELISA

Indexed keywords

ALLERGEN; IMMOBILIZED ANTIBODY; MONOCLONAL ANTIBODY; PEROXIDASE; POLLEN ANTIGEN; STREPTAVIDIN;

ACCURACY; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; BIOTINYLATION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GRASS POLLEN; HYBRIDOMA; IMMUNIZATION; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ALLERGENS; ANIMALS; ANTIBODIES, MONOCLONAL; BINDING, COMPETITIVE; CROSS REACTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; LOLIUM; MICE; MICE, INBRED BALB C; MOLECULAR WEIGHT; PLANT PROTEINS; POACEAE; POLLEN;

EID: 0035726391     PISSN: 09547894     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2222.2001.01166.x     Document Type: Article

References (37)

1. Identification of a 36 kDa olive-pollen allergen by in vitro and in vivo studies
2. Antigenic and structural analysis of group II allergens (Der f II and Der p II) from house dust mites (Dermatophagoides spp)
3. Monoclonal antibodies to group II Dermatophagoides spp. allergens: Murine immune response, epitope analysis, and development of a two-site ELISA
4. A new start for allergen references and standardization based on purified (natural or recombinant) allergens and monoclonal and monospecific polyclonal antibodies
5. What are the important allergens in grass pollen that are linked to human allergic disease?
6. How far can we simplify in vitro diagnostics for grass pollen allergy? A study with 17 whole pollen extracts and purified natural and recombinant major allergens
7. A two-site monoclonal antibody ELISA for the quantification of group V allergens in grass extracts
8. Group 5 determination in Pooideae grass pollen extracts by monoclonal antibody-based ELISA. Correlation with biologic activity
9. Detection and quantification of group 4 allergens in grass pollen extracts using monoclonal antibodies
10. Concentrations of major grass group 5 allergens in pollen grains and atmospheric particles: Implications for hay fever and allergic asthma sufferers sensitized to grass pollen allergens
11. cDNA cloning and immunological characterization of the rye grass allergen Lol p I
12. DNA sequencing with chain-terminating inhibitors
13. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion
14. Application of ELISA inhibition to Aspergillus antigen standardization for immunodiagnosis
15. 4
16. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
17. Cloning and sequencing of Lol p I, the major allergenic protein of rye-grass pollen
18. Isolation and characterization of Poa p I allergens of Kentucky bluegrass pollen with a murine monoclonal anti-Lol p 1 antibody
19. Characterization of grass group I allergens in timothy grass pollen
20. Purification and characterization of a major allergen from Dactylis glomerata pollen. The Ag Dg 1
21. Quantification in mass units of Bet v 1, the main allergen of Betula verrucosa pollen, by a monoclonal antibody based ELISA
22. Quantitation of the major allergen of several Parietaria pollens by an anti-Par 1 monoclonal antibody-based ELISA. Analysis of crossreactivity among purified Par j 1, Par o 1 and Par m 1 allergens
23. Monoclonal antibodies against Olea europaea major allergen: Allergenic activity of affinity-purified allergen and depleted extract and development of a radioimmunoassay for the quantitation of the allergen
24. Monoclonal immunoassays for major dust mite (Dermatophagoides) allergens, Der p I and Der f I, and quantitative analysis of the allergen content of mite and house dust extracts
25. Monoclonal antibodies to the major feline allergen Fel d I. II. Single step affinity purification of Fel d I, N-terminal sequence analysis, and development of a sensitive two-site immunoassay to assess Fel d I exposure
26. Environmental exposure to cockroach allergens: Analysis with monoclonal antibody-based enzyme immunoassays
27. Biochemical and immunological characterization of recombinant allergen Lol p 1
28. Analytic aspects of the standardization of allergenic extracts
29. The use of standardized allergen extracts
30. A sensitive monoclonal antibody sandwich ELISA for the measurement of the major olive pollen allergen Ole e 1
31. Determination of Ole e 1 by enzyme immunoassay and scanning densitometry. Validation by skin-prick testing
32. How far can we simplify in vitro diagnostics for Fagales tree pollen allergy? A study with three whole pollen extracts and purified natural and recombinant allergens
33. Purified natural and recombinant Fel d 1 and cat albumin in in vitro diagnostics for cat allergy
34. Clinical testing of recombinant allergens of the mold Alternaria alternata
35. Allergen immunotherapy: Therapeutic vaccines for allergic diseases
36. Long-term clinical efficacy of grass-pollen immunotherapy