Gag-derived proteins of HIV-1 isolates from Indian patients: Cloning, expression, and purification of P17 of B- and C-subtypes

Protein Expression and Purification

Volumn 21, Issue 3, 2001, Pages 378-385

  Gupta, Sanjay ^a   Arora, Kajal ^a   Gupta, Amita ^a   Chaudhary, Vijay K ^a  

^a UNIVERSITY OF DELHI   (India)

Author keywords

Gag sequencing; HIV 1 gag; HIV 1 p17; HIV 1 subtypes; Protein expression; Protein purification

Indexed keywords

ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0035718924     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1389     Document Type: Article

References (30)

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2. HIV-1 strains from India are divergent from prototypic African and US/European strains, but are linked to a South African isolate
3. Genetic analysis of human immunodeficiency virus type 1 and 2 (HIV-1 and HIV-2) mixed infections in India reveals a recent spread of HIV-1 and HIV-2 from a single ancestor for each of these viruses
4. Decline of anti-p24 antibody precedes antigenaemia as correlate of prognosis in HIV-1 infection
5. Overproduction, purification and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24 and p15
6. Prevalence of antibodies to the core protein p17, a serological marker during HIV-1 infection
7. Decline of antibody reactivity to outer viral core protein p17 is an earlier serological marker of disease progression in human immunodeficiency virus infection than anti-p24 decline
8. Shortening of the window period in diagnosis of HIV-1 infection by simultaneous detection of p24 antigen and antibody IgG to p17 and reverse transcriptase in serum with ultrasensitive enzyme immunoassay
9. Structure-function studies of the human immunodeficiency virus type 1 matrix protein, p17
10. Binding of human immunodeficiency virus type 1 Gag to membrane: Role of the matrix amino terminus
11. Direct interaction between the envelope and matrix proteins of HIV-1
12. Genetic evidence for an interaction between human immunodeficiency virus type 1 matrix and alpha-helix 2 of the gp41 cytoplasmic tail
13. Domains of the human immunodeficiency virus type 1 matrix and gp41 cytoplasmic tail required for envelope incorporation into virions
14. Role of the karyopherin pathway in human immunodeficiency virus type 1 nuclear import
15. Backbone cyclic peptide, which mimics the nuclear localization signal of human immunodeficiency virus type 1 matrix protein, inhibits nuclear import and virus production in nondividing cells
16. Human immunodeficiency virus type 1 matrix protein interacts with cellular protein HO3
17. Human immunodeficiency virus matrix tyrosine phosphorylation: Characterization of the kinase and its substrate requirements
18. cDNA encoding a single-chain antibody to HIV p17 with cytoplasmic or nuclear retention signals inhibits HIV-1 replication
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23. An expression system for secretion and purification of a genetically engineered thermostable chimera of protein A and alkaline phosphatase
24. Use of T7 RNA polymerase to direct expression of cloned genes
25. Rapid and efficient site-specific mutagenesis without phenotypic selection
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27. Complete nucleotide sequence of the AIDS virus HTLV-III
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