Pronuclear microinjection

Cloning and Stem Cells

Volumn 3, Issue 4, 2001, Pages 209-220

  Wall, Robert J ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONFERENCE PAPER; EMBRYO TRANSFER; GENE NUMBER; GENE TRANSFER; MICROINJECTION; MOLECULAR CLONING; PRIORITY JOURNAL; PRONUCLEUS; TRANSGENIC ANIMAL;

ANIMALIA; MAMMALIA;

EID: 0035704592     PISSN: 15362302     EISSN: None     Source Type: Journal    
DOI: 10.1089/15362300152725936     Document Type: Conference Paper

References (89)

1. Expression of a foreign gene in a line of transgenic mice is modulated by a chromosomal position effect
2. DNA hybrids stabilized by heterologies
3. Mechanism of chromosomal integration of microinjected DNA
4. Restriction enzyme-mediated integration elevates transformation frequency and enables co-transfection of Toxoplasma gondii
5. Production of bovine alpha-lactalbumin in the milk of transgenic pigs
6. Positive selection of transgenic bovine embryos in culture
7. Transgenic livestock
8. Production of transgenic cattle by pronuclear injection
9. Patterns of expression of position-dependent integrated transgenes in mouse embryo
10. Uptake of hetrolougous genome by mammalian spermatozoa and its transfer to ova through fertilization
11. Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines
12. Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs
13. Fate of microinjected genes in preimplantation mouse embryos
14. High-efficiency transformation by direct microinjection of DNA into cultured mammalian cells
15. Transgenic cattle produced by reverse-transcribed gene transfer in oocytes
16. Timing of DNA integration, transgenic mosaicism, and pronuclear microinjection
17. A 5′ element of the chicken beta-globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila
18. Cloned transgenic calves produced from non-quiescent fetal fibroblasts
19. Generation of transgenic mice and germline transmission of a mammalian artificial chromosome introduced into embryos by pronuclear microinjection
20. Introduction of a rabbit beta-globin gene into the mouse germ line
21. Mammalian artificial chromosome pilot production facility: Large-scale isolation of functional satellite DNA-based artificial chromosomes
22. Microinjecting DNA into mouse ova to study DNA replication and gene expression and to produce transgenic animals
23. Methods for micromanipulation of human somatic cells in culture
24. Transgenic cow production from microinjection into in vivo-derived embryos
25. Episomal maintenance of a bovine papilloma virus vector in transgenic mice
26. Production of transgenic cattle expressing human alpha-lactalbumin in milk
27. High-avidity human IgG kappa monoclonal antibodies from a novel strain of minilocus transgenic mice
28. Effect of microinjection time during postfertilization S-phase on bovine embryonic development
29. Repeat-induced gene silencing in mammals
30. Size matters: Use of YACs, BACs and PACs in transgenic animals
31. Integration and stable germ line transmission of genes injected into mouse pronuclei
32. Genetic transformation of mouse embryos by microinjection of purified DNA
33. Retransformation of a simian virus 40 revertant cell line, which is resistant to viral and DNA infections, by microinjection of viral DNA
34. Transformation of rat cells by DNA of human adenovirus 5
35. Effect of flanking matrix attachment regions on the expression of microinjected transgenes during preimplantation development of mouse embryos
36. Use of a short-life green fluorescent protein to predict transgene integration in microinjected mouse embryos
37. Production of transgenic rabbits, sheep and pigs by microinjection
38. Production of transgenic cattle by pronuclear injection
39. A comparative study on the integration of exogenous DNA into mouse, rat, rabbit, and pig genomes
40. In vitro selection of transgenic mouse embryos under the presence of puromycin
41. Germ-line integration and Mendelian transmission of the exogenous Moloney leukemia virus
42. Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA
43. Production of a mutation in mouse En-2 gene by homologous recombination in embryonic stem cells
44. Effect of short interspersed element sequences on the integration and expression of a reporter gene in the preimplantation-stage mouse embryos
45. Preimplantation screening for transgenesis using an embryonic specific promoter and green flourescent protein
46. Double pronuclei injection of DNA into zygotes increases yields of transgenic mouse lines
47. Sperm cells as vectors for introducing foreign DNA into eggs: Genetic transformation of mice
48. Homology-directed repair is a major double-strand break repair pathway in mammalian cells
49. Recombination in mouse L cells between DNA introduced into cells and homologous chromosomal sequences
50. Mutagenesis via insertional- or restriction enzyme-mediated-integration (REMI) as a tool to tag pathogenicity related genes in plant pathogenic fungi
51. Sperm-mediated gene transfer in mice
52. The isolation of structural genes from libraries of eucaryotic DNA
53. Nonhomologous end joining during restriction enzyme-mediated DNA integration in Saccharomyces cerevisiae
54. Gene targeting in murine embryonic stem cells: Introduction of specific alterations into the mammalian genome
55. Production of transgenic swine
56. Matrix-attachment regions can impart position-independent regulation of a tissue-specific gene in transgenic mice
57. The difference in expression pattern of green fluorescent protein after gene microinjection into mouse zygotes and two-cell embryos
58. Prediction of transgene integration by noninvasive bioluminescent screening of microinjected bovine embryos
59. Production of transgenic merino sheep by microinjection of ovine metallothionein-ovine growth hormone fusion genes
60. Production of a sheep transgenic with the ovine growth hormone gene
61. Genetic applications of an inverse polymerase chain reaction
62. Xenopus laevis TRK-fused gene (TFG) is an SH3 domain binding protein highly expressed in the cement gland
63. Germline transformation of mice
64. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes
65. Metallothionein-human growth hormone fusion genes stimulate growth of mice
66. Efficient generation of functional transgenes by homologous recombination in murine zygotes
67. Effect of flanking matrix attachment regions on the expression of microinjected transgenes during preimplantation development of mouse embryos
68. Effect of three types of repetitive sequences in mouse transgene intetration
69. Development of 1-cell and 2-cell pig ova after microinjection of genes
70. Effects of transferred ova per recipient and dual use of donors as recipients on production of transgenic swine
71. Effects of transfer conditions on production of transgenic pigs
72. Transfer of an ovine metallothionein-ovine growth hormone fusion gene into swine
73. Sequence and chromosomal context effects on variegated expression of keratin 5/lacZ constructs in stratified epithelia of transgenic mice
74. Use of bovine satellite sequences to increase transgene integration by homologous recombination in bovine embryos
75. Enhanced homologous recombination
76. Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae
77. Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts
78. Co-injection of restriction enzyme with foreign DNA into the pronucleus for elevating production efficiencies of transgenic animals
79. Gene transfer into sheep
80. Homologous recombination between defective neo genes in mouse 3T6 cells
81. Position-independent and copy-number-related expression of a goat bacterial artificial chromosome alpha-lactalbumin gene in transgenic mice
82. Association of the 5′HS4 sequence of the chicken beta-globin locus control region with human EF1 alpha gene promoter induces ubiquitous and high expression of human CD55 and CD59 cDNAs in transgenic rabbits
83. Selective production of transgenic mice using green fluorescent protein as a maker
84. High frequency targeting of genes of specific sites in the mammalian genome
85. Real time imaging of transcriptional activity in live mouse preimplantation embryos using a secreted luciferase
86. Manipulating the mammalian genome by homologous recombination
87. DNA preparation method can influence outcome of transgenic animal experiments
88. Development of porcine ova that were centrifuged to permit visualization of pronuclei and nuclei
89. Production of chimaeric mice containing embryonic stem (ES) cells carrying a homoeobox Hox 1.1 allele mutated by homologous recombination