Skin-targeted gene transfer using in vivo electroporation

Gene Therapy

Volumn 8, Issue 23, 2001, Pages 1808-1812

  Maruyama, H ^a   Ataka, K ^a   Higuchi, N ^a   Sakamoto, F ^a   Gejyo, F ^a   Miyazaki, J ^a  

^a NIIGATA UNIVERSITY   (Japan)

Author keywords

Electroporation; Erythropoietin; Gene transfer; Skin

Indexed keywords

BETA GALACTOSIDASE; ERYTHROPOIETIN; MESSENGER RNA; PLASMID DNA; PLASMID VECTOR;

ABDOMEN; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DOSE RESPONSE; ELECTRIC POTENTIAL; ELECTROPORATION; EPIDERMIS; GENE EXPRESSION; GENE TRANSFER; HEMATOCRIT; IN VIVO STUDY; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; RAT; SKIN DEFECT; SKIN ELECTRODE; SUBCUTANEOUS TISSUE;

ANIMALS; ELECTROPORATION; ERYTHROPOIETIN; GENE TARGETING; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; MALE; RATS; RATS, WISTAR; SKIN;

ANIMALIA;

EID: 0035702872     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301604     Document Type: Article

References (18)

1. Cytokine gene expression in epidermis with biological effects following injection of naked DNA
2. Expression of naked DNA in human, pig, and mouse skin
3. The feasibility of targeted selective gene therapy of the hair follicle
4. A direct in vivo approach for skin gene therapy
5. Direct gene transfer into mouse muscle in vivo
6. Intradermal gene immunization: The possible role of DNA uptake in the induction of cellular immunity to viruses
7. Immunization via hair follicles by topical application of naked DNA to normal skin
8. Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles
9. Vivo promoter activity and transgene expression in mammalian somatic tissues by using particle bombardment
10. Vivo gene introduction into keratinocytes using jet injection
11. Safe and effective regulation of hematocrit by gene gun administration of an erythropoietin-encoding DNA plasmid
12. Vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA
13. Depth-targeted efficient gene delivery and expression in the skin by pulsed electric fields: An approach to gene therapy of skin aging and other diseases
14. Long-term production of erythropoietin after electroporation-mediated transfer of plasmid DNA into the muscles of normal and uremic rats
15. Requiring higher doses of erythropoietin suggests pregnancy in hemodialysis patients
16. Continuous erythropoietin delivery by muscle-targeted gene transfer using in vivo electroporation
17. Efficient selection for high-expression transfectants with a novel eukaryotic vector
18. Gene transfer into muscle by electroporation in vivo