Development of a genomic in situ hybridization method using Technovit 7100 sections of early wheat embryo

Biotechnic and Histochemistry

Volumn 76, Issue 5-6, 2001, Pages 257-260

  Mochida, K ^a   Tsujimoto, H ^a  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

GISH; Glycol methacrylate resin; Super wide cross; Technovit 7100; Triticum aestivum

Indexed keywords

MESSENGER RNA; PLANT DNA; RESIN; WATER;

ARTICLE; AUTOFLUORESCENCE; CHROMOSOME; CONTROLLED STUDY; DNA DENATURATION; FLUORESCENCE MICROSCOPY; GENOMIC IN SITU HYBRIDIZATION; HISTOCHEMISTRY; LEYMUS MOLLIS; MAIZE; NONHUMAN; PLANT; PLANT GENETICS; PLANT OVARY; WATER PERMEABILITY; WHEAT;

LEYMUS; LEYMUS MOLLIS; TRITICUM; TRITICUM AESTIVUM; ZEA MAYS;

EID: 0035691886     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.1080/714028159     Document Type: Article

References (12)

1. Studies on the behavior of organelles and their nucleotides in the root apical meristem of Arabidopsis thariana (L.) Col
2. Changes in cytoplasmic rRNA density in the root apical meristem observed by high resolution in situ hybridization
3. Uniparental chromosome elimination in the inviable salmonid hybrids between masu salmon female and rainbow trout male
4. Studies on the behaviour of mitocondrial DNA. Synthesis of mitocondrial DNA occurs actively in a specific region just above the quiescent centre in the root meristem of Pelargonium zonale
5. Application of embedding of samples in Technovit 7100 resin to observations of small amounts of DNA in the cellular organelles associated with cytoplasmic inheritance
6. Fluorescence microscopic study of the formation of giant mitochondria nuclei in the young ovules of Pelargonium zonale
7. The timing of chromosome some elimination in haploid wheat-maize crosses
8. In situ hybridization
9. Molecular identification of the D genome chromosome of wheat
10. Extent of the condensation of nuclear chromatin and the localization of RNA during pollen development in Nicotiana tabacum
11. Detection and quantification of rRNA by high-resolution in situ hybridization in pollen grains
12. Improved sensitivity for high resolution in situ hybridization using resin extraction of methyl methacrylate embedded material